摘要
目的:测定明胶和吸收性明胶海绵的总蛋白质含量,为控制和提高产品质量提供依据。方法:分别用双缩脲法、联喹啉二羧酸法和考马斯亮蓝法测定了明胶和吸收性明胶海绵的总蛋白质含量。结果:考马斯亮蓝G250与明胶溶液的显色反应不够灵敏,而与吸收性明胶海绵的水解液不显色。双缩脲法和2,2′-联喹啉-4,4′二羧酸法的测定结果分别为70%～78%和51%～64%。采用上述同一种方法分别测定明胶和吸收性明胶海绵的总蛋白质含量,结果无显著性差异。结论:考马斯亮蓝法不适用于明胶和吸收性明胶海绵的总蛋白质含量测定,而双缩脲法和2,2′-联喹啉-4,4′二羧酸法的测定结果具有参考价值。
Objective：The total proteins contents of gelatin samples and gelatin sponge samples are meant to known.Method：The total proteins contents of gelatin samples and gelatin sponge samples are determined by Biuret＇s agent,4,4′-dicarboxy-2,2′-biquinolin and coomassie brilliant blue G-250,respectively.Result：The results of Biuret＇s method,70%-78%,are greater by about 20 percent points than BCA method,51%-64%.The total proteins contents of gelatin samples and gelatin sponge samples are similar when using the same method.Conclusion：Bradford＇s method seems unfeasible to gelatin samples and gelatin sponge samples due to the insensitive coloration reaction of coomassie brilliant blue G-250 with samples.Biuret＇s method and BCA method could be applied for the determination.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第8期1563-1566,共4页
Chinese Journal of Pharmaceutical Analysis
