摘要
本文以碳-13富集的甲醛为交联剂,用核磁共振波谱法对甲醛和明胶的交联反应进行了直接跟踪和详尽研究。使此反应的过程,交联反应发生的方位及其结果得到了更为客观的认识。结果表明,和蛋白质反应的主要成分是甲醛单体(水合物)而非聚合物。其次,交联反应所形成的亚甲基桥是在赖氨酸(或羟基赖氨酸＊)的侧链氨基和精氨酸的侧链胍基之间,反应属典型的曼尼克反应(Mannich Reaction)。而不是在赖氨酸的侧链氨基之间。此反应也有少量精氨酸胍基的羟亚甲基衍生物生成。(＊以下只用赖氨酸来代替二者)
Current research has focused on direct studies of crosslinking rea-
ction of gelatin with ^(13)C-enriched formaldehyde by means of Nuclear
Magnetic Resonance (NMR) spectroscopy, revealing the crosslinking
reaction sites in protein, process and the reaction results. The results
of this study have shown that it is the monomer(hydrate) of formalde-
hyde which reacts with gelatin but not the polymers. Additionally, the
reaction forms crosslinks in the from of -CH_2- between amino groups
of lysine (or hydroxy-lysine) residues and the guanidine groups of argini-
ne.This reaction is typical Mannich Reaction.There is also a little hpdro-
xymethylene derivatives of arginine formed.No participation of peptide bond
or amide group has been found.
出处
《中国皮革》
CAS
1993年第10期43-46,共4页
China Leather
关键词
核磁共振波谱
甲醛
胶原
交联
Formaldehyde Gelatin Crosslinking Reaction NMR Formaldehyde Tannage Mannich Reaction
