摘要
目的:建立一种血红蛋白氧载体中巯基抗氧化剂的方法。方法:研究发现巯基抗氧化剂和聚合血红蛋白之间有一定的吸附作用并且难以分离,因此以血红蛋白为对照品,邻二氮菲-Fe3+为显色剂和氧化剂,在510nm处直接测定分别含有半胱氨酸、谷胱甘肽和氮-乙酰-半胱氨酸的3种血红蛋白氧载体溶液中这3种巯基抗氧化剂的含量。结果:该方法在1×10-6～1.5×10-5mol.L-1范围内有良好的线性关系,相关系数分别为0.9991,0.9989,0.9985,RSD分别为4.1%,3.4%,3.9%,回收率分别为97.3%±3.5%,96.7%±4.1%,95.5%±4.3%。结论:该方法避免了聚合血红蛋白与巯基抗氧化剂的分离并可直接测定血红蛋白氧载体中巯基抗氧化剂含量,是一种简便、准确、回收率高的方法。
Objective：To establish a method for determination of thiol anti-oxidant in Hemoglobin-Based Oxygen carrier（HBOC）.Method：It is found that polymerized hemoglobin in HBOC can absorb the thiol anti-oxidant which is difficult to be separated,so taking the hemoglobin as the control and o-phenanthroline-Fe3＋ as developer and oxidant,direct determining the content of cysteine,glutathion and N-acetyl-cysteine in HBOC at 510 nm.Results：all the calibration curves showed good linearity in the range of 1×10-6-1.5×10-5 mol·L-1,and the correlation coefficient are 0.9991,0.9989 and 0.9985,all of them have the good precision,and the recovery are 97.3%±3.5%,96.7%±4.1% and 95.5%±4.3%,respectively.Conclusion：This method avoided separating the thiol anti-oxidant from the HBOC,and can direct determine the contents of thiol anti-oxidant.It is a simple,accurate,high recovery method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第8期1533-1536,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家"863计划"(No.2006AA02A143)
陕西省教育厅专面科研计划项目(09JK784)
