摘要
研究了邻二氮菲-Fe3+与半胱氮酸的反应,发现半胱氨酸浓度在1.0×10-5~1.8×10-4mol.L-1范围内其产物吸光度值符合朗伯比耳定律。检测限1.0×10-6mol.L-1,表观摩尔吸光系数7.9×103L.mol-1.cm-1。采用本法测定谷氨酸、赖氨酸、半胱氨酸混合试样中半胱氨酸含量,相对标准偏差≤2%,结果令人满意。
The article has researched the reaction of mixing o-phenanthroline and cysteine up. It has been found that absorbanee of the production is in line with Beris low when the concentration of eysteine varies from 1.0 × 10 ^-5 - 1.8 × 10^ -4mol · L^-1. The minimum concentration detected is 1.0 × 10^-6mol · L^-1. To determine the eysteine Glutamie aerd Lysine o-phenanthroline and eysteine with this method, the relative meansquare deviation is equal to 2% or less than it. The result is satisfying.
出处
《信阳农业高等专科学校学报》
2006年第3期131-132,共2页
Journal of Xinyang Agricultural College
关键词
邻二氮菲-Fe^3+
光度法
半胱氨酸
o-phenanthroline - Fe^3 ＋
absorption speetrophotometery
Cysteine
