摘要
目的探讨骨形成蛋白-7(BMP-7)在铜绿假单胞菌(PAE)诱发大鼠慢性肺部感染而致气道重构中的作用。方法随机分为生理盐水对照组(NS组)和铜绿假单胞菌感染组(PAE组)各60只,通过多次经气管穿刺注入一定剂量PAE菌液,建立大鼠慢性肺部感染模型;观察气管及肺组织的病理改变,测定气管壁和血管壁厚度,并采用实时定量RT-PCR方法检测肺组织中BMP-7 mRNA的表达,分析BMP-7表达与病理改变的相关性。结果大鼠感染PAE第4周开始,气管壁厚度和血管壁厚度均较NS组显著增加,差异有统计学意义(P<0.05),至第16周时,支气管管壁厚度与血管壁厚度达最大值,分别为(524.5±18.6)(、163.3±12.5)μm,管腔明显狭窄,有气道重构和肺气肿形成;而BMP-7表达随着时间的延长逐渐减弱,和对照组相比,差异有统计学意义(P<0.01);BMP-7表达与气管壁厚度和血管壁厚度呈显著负相关,相关系数分别为-0.884,-0.775,差异有统计学意义(P<0.01)。结论反复气道感染铜绿假单胞菌可致大鼠出现气道重构,而BMP-7可能参与了气道重构的发生和发展过程。
OBJECTIVE To study the role of bone morphogenetic protein-7（BMP-7） played on airway remodeling of chronic pulmonary infection rats induced by Pseudomonas aeruginosa.METHODS P.aeruginosa had been injected into trachea for several times by trachea puncturing.Pathology changes of trachea and lung were observed,and thickness of trachea wall and vessel wall were measured.At the same time,methods of real-time RT-PCR was adopted to determine mRNA expression of BMP-7 of lung tissue.And then,the relation between pathologic changes and BMP-7 were also analysis.RESULTS From the 4th week,the thickness of trachea wall and vessel wall were more thicker than that of NS group（P0.05）,the figure were 524.5±18.6 um and 163.3±12.5 um respectively at the 16th week.There appeared obvious chronic inflammation in all grade bronchis,which trachea walls were thickened and lumens were narrowed.while the expression of BMP-7 was significantly inhibited（P0.01）which was negative associated with thickness of trachea wall and vessel wall respectively（r=-0.884,-0.775,P0.01）.CONCLUSION Airway remodeling of rats can be induced by repeated injecting P.aeruginosa and BMP-7 may play a part role on it.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第15期3125-3128,共4页
Chinese Journal of Nosocomiology
基金
福建省自然科学基金计划项目(Z0516077)
关键词
气道重构
铜绿假单胞菌
骨形成蛋白-7
Airway remodeling
Pseudomonas aeruginosa
Bone morphogenetic protein-7
