摘要
目的探讨铜绿假单胞菌(Pseudomonas aeruginosa,PA)群体感应系统(quorum sensing system,QS)对CD4+CD25+Treg细胞的影响及作用机制。方法60只雄性SD大鼠,随机分为铜绿假单胞菌野生菌株PAO1组,变异菌株PAO-JP2组(lasI、rhlI基因双变异)和空白对照组。将PA包被的硅胶管置入大鼠一侧主支气管建立慢性肺部感染模型,对照组置入无菌硅胶管。28 d后,FACs测外周血CD4+CD25+Treg细胞数量,ELISA测血清IL-10、TGF-β,RT-PCR测脾Foxp3mRNA表达水平,肺组织行HE染色。结果CD4+CD25+Treg/CD4+T淋巴细胞的百分比:PAO1组(19.79±6.45)%,PAO-JP2组(11.03±3.92)%,对照组(5.15±0.47)%,PAO1组的百分比明显高于PAO-JP2组和对照组(P<0.05)。IL-10:PAO1组(231.52±54.48)pg/mL,PAO-JP2组(60.10±29.64)pg/mL,对照组(35.43±23.56)pg/mL,PAO1组表达水平高于JP2、对照组(P<0.05)。TGF-β:PAO1组、JP2组、对照组水平分别为(121.05±7.98)pg/mL、(63.11±5.73)pg/mL、(36.02±8.94)pg/mL,PAO1组与PAO-JP2、对照组比较均有统计学意义。Foxp3mRNA相对含量:PAO1组(0.80±0.044),PAO-JP2组(0.41±0.044),对照组(0.25±0.054),PAO1组较其他两组含量显著升高(P<0.05)。HE染色可见,PAO1组肺组织大量淋巴细胞聚集、纤维增生明显,脓肿形成;PAO-JP2组炎症细胞浸润叫PAO1组明显减轻,对照组未见明显异常。结论PA群体感应系统通过提高IL-10、TGF-β和Foxp3mRNA的表达水平,上调CD4+CD25+Treg细胞的数量及功能,在慢性肺部感染中发挥重要作用。
Objective To explore the effects of quorum-sensing system on CD4+CD25+ Treg cell in Pseudomonas aeruginosa.Methods Sixty SD rats were divided into 3 groups: PAO1 group,PAO-JP2(lasI and rhlI double mutant) group,and control group.Chronic lung infection model was set up by placing P.aeruginosa-precoated silicone tube into the main bronchus.Twentyeight days later,The Treg cell in venous blood,the IL-10 and TGF-β in serum,and the Foxp3mRNA in spleen were detected by FACs,ELISA,and RT-PCR,respectively.The pulmon...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第4期402-405,共4页
Immunological Journal
