摘要
研究了一种用于临床检测血清蛋白的毛细管区带电泳方法。弹性石英毛细管５０ μｍ ｉ．ｄ．×４７ ｃｍ （４０ ｃｍ 有效长度），检测波长２００ ｎｍ ，血清用运行缓冲液（含１２．５ ｍ ｍ ｏｌ／Ｌ四硼酸钠、１ ｍ ｍ ｏｌ／Ｌ乳酸钙、０．７ ｍ ｍ ｏｌ／Ｌ硫酸镁，ｐＨ ９．７０）稀释４０倍，气压进样１７．２３ ｋＰａ·ｓ，分析电压２３ ｋＶ。正常血清蛋白分为６种，孕妇的分７种（多一个未知的α０峰）。将正常人、孕妇、多发性骨髓瘤和强直性脊柱炎患者的血清蛋白的毛细管电泳与传统的醋酸纤维膜电泳相比较，前者具有高分辨率、在线数据处理和自动化的特点。基于血清蛋白的诊断，毛细管电泳是一个可靠的分析新技术。
The separation method of serum proteins was established with an untreared 50 μm i.d.×47 cm (40 cm to detector) capillary and detection of absorbance at 200 nm.Analysis was performed by pressure injectction 17.23 kPa·s and by applying 23 kV in the constant voltage mode. Serum samples were diluted 40\|folds with assay buffer (12.5 mmol/L sodium borate,1 mmol/L calcium lactate,0.7 mmol/L magnesium sulfate,1 mmol/L EDTA were mixed). A normal control serum protein was separated into 6 fractions. In pregnant serum, the α 0 was an additionally unknown fraction.Comparison of capillary electrophoresis with conventional cellulose acetate electrophoresis for analysis of serum proteins from normal control,pregnant women multiple myeloma and tonic rachitis patients indicates that capillary clectrophoresis is a new technique for the analysis of serum proteins because of its high efficiency,on\|line data processing and automation. Capillary electrophoresis is the reliable technique for clinical diagnosis of serum protein abnormalities.
出处
《色谱》
CAS
CSCD
北大核心
1999年第6期593-595,共3页
Chinese Journal of Chromatography
