摘要
连续4 a调查了以袋繁和自繁方式生产的4个滇Ⅰ型不育系的11个群体,发现"可育株"的出现频率为0.087 1%,其表型与相应保持系一致。采用测交和分子标记技术分析了这些"可育株"的基因型,发现袋繁不育系群体存在核恢复基因位点基因型为rf/rf的"保持株"和Rf/rf的"回复株"2种类型,而自繁不育系群体还新出现基因型为RfRf的"纯合株"类型。与袋繁群体比,自繁群体出现"可育株"的频率提高了74.7%,其中"保持株"频率提高了42.6%,"回复株"频率提高了149.3%。基于不育系繁殖方法不同,不育系中出现"可育株"混杂的几率不同,为获得高纯度的不育系种子,滇I型不育系繁殖应在袋繁基础上,扩大自繁群体,减少自繁次数。
It was found that the ratio of fertile plants（FP） was 0.087 1% on average based on the investigation of 11 populations of four japonica Dian I-type CMS Lines（CMS-D1） generated by bagged pollination multiplication（BPM） and natural pollination multiplication（NPM） for four years（2005—2008）,and the phenotype of fertile plants was similar to that of maintainer plants.The analysis based on selfing,testcrossing and DNA marker techniques showed that there were two genotypes,i.e.,rf/rf（maintainer plants） and Rf/rf（revertants）,for the fertile plants in the populations generated by BPM,but in the populations generated by NPM,besides the above two genotypes,a new genotype,Rf/Rf（homozygous plants）,was found.In comparison with the BPM populations,the NPM populations had a higher ratio of fertile plants by 74.7%,of maintainer plants by 42.6% and of revertants by 149.3%.Since different methods of multiplication results in a different degree of impurity of fertile plants in CMS populations,it is necessary to enlarge the size of NPM population on the basis of BPM and lessen the multiplying times to ensure a high seed purity in the multiplication of CMS-D1.
出处
《杂交水稻》
CSCD
北大核心
2011年第4期55-60,共6页
Hybrid Rice
基金
教育部博士点基金项目(20070676001)
云南省科技攻关项目(2009BB007)
博士启动经费(A2002171)
科技成果转化项目(2009GB2F300346)
关键词
水稻滇Ⅰ型不育系
袋繁
自繁
可育株频率
可育株基因型
Rice
Dian I-type CMS Line
bagged pollination multiplication
natural pollination multiplication
frequency of fertile plants
genotype of fertile plants
