摘要
以嗜热链球菌为实验菌株,利用摇床液体发酵,采用完全随机化设计研究不同培养温度、不同培养时间和植物(枸杞和刺五加)提取液不同添加量对嗜热链球菌产超氧化物歧化酶(SOD)的影响,并用方差分析和回归分析对不同处理的实验结果进行比较。方差分析表明:不同培养温度对嗜热链球菌产SOD的影响差异极显著(P<0.01),当培养温度达到41℃时,SOD平均产量达到最高(901.667U/g);不同培养时间对嗜热链球菌产SOD的影响差异极显著(P<0.01),当培养时间为48h时,SOD平均产量达到最高(932.6667U/g);不同植物提取液添加量对嗜热链球菌产SOD的影响差异极显著(P<0.01),植物提取液添加量为8%时,SOD平均产量达到最高(1347.0000U/g)。综合考虑降低成本,提高效益等因素,确定最优方案为:培养温度41℃、培养时间24h、植物提取液添加量为8%,此时获得较高的SOD产量,且投入与产出比较高。回归分析表明:SOD产量与培养温度之间回归关系不显著(P>0.05);SOD产量与植物提取液添加量之间存在极显著的线性回归关系:Y=865.033+52.383X(P<0.01);SOD产量与培养时间之间存在极显著的线性回归关系:Y=238.757+17.346X(P<0.01)。
The effects of temperature,culture time and plant extract,an aqueous extract obtained from mixed Lycium barbarum and Eleutherococcus senticosus at a ratio of 1：1（m/m） and concentrated by rotary evaporation until a concentration of 1.0 g/mL（total material weight/concentrated extract solution volume ratio） on the production of superoxide dismutase（SOD） by liquid shake-flask fermentation of Streptococcus thermophilus were studied using completely randomized design.Experimental data processing was carried out using analysis of variance（ANOVA） and regression analysis.The ANOVA results showed that： 1）.The effect of culture temperature on SOD production was highly significant（P0.01）;when the culture temperature was 41 ℃,the highest SOD yield was 901.667 U/g wet cells.2）.The effect of culture time on SOD production was also highly significant（P0.01）;when the culture time was 48 h,the highest yield of SOD was 932.6667 U/g wet cells.3）.The effect of plant extracts on SOD production was significant（P0.01）;when plant extract addition was at 8%,the highest SOD yield was 1347.0000 U/g wet cells.Therefore,the optimal culture conditions that can maximize SOD yield and result in a higher input-to-output ratio were culture temperature at 41 ℃,fermentation duration of 24 h,and 8% plant extract.The regression analysis showed no significant correlation between SOD production and culture temperature（P0.05）.SOD production（Y） showed a highly significant linear relationship with plant extract amount or culture time,and the corresponding regression equations were Y = 865.033 ＋ 52.383X（P0.01） and Y = 238.757 ＋ 17.346X（P0.01）,respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第15期173-176,共4页
Food Science
基金
2009年山西省高校科技开发项目(20091002)
关键词
嗜热链球菌
超氧化物歧化酶
培养条件
Streptococcus thermophilu
superoxide dismutase
NBT reduction
culture conditions
