摘要
建立成体鸟纲与哺乳纲两种动物初级听觉神经元的体外模型并观察其存活与发育的细胞生物学特征。对出生后１０－１６日龄的ＷＬＳ幼鸡和７日龄的Ｗｉｓｔａｒ大鼠初级听觉神经进行有血清原代培养，倒置相差镜与荧光显微镜下观察其细胞活力与发育过程。应用链霉卵白素／过氧化物酶方法和抗神经丝蛋白单克隆抗体进行免疫细胞化学鉴定。
In order to understand the cytobiological features of primary auditory neurons invitro, the culture model of auditory neuron were created with serum element from postnatal 10~16 day WLS chicken and 7 days postnata1 (P7) Wistar rats. We observed the cellular growth ofauditory nerve by inverted/phase contrast and fluoresent microscope and particularly describedtheir cytobiological character of development and differentiation process. Immunocytochemicl iden-tification was carried out on the cultured spiral ganglion neuron (SGN) of P7 Wistar rats by meansof S-P method and monoclonal antibody of neurofiament protein (Anti-NFP-McAb) to show thedegree of phenotypic differentiation. Our result demonstrated that the ganglion and dissocialedneurons frorn the two postnatal species could survive well and differentiate to a normal phenotypicfeature, the surviving period and cell amount of auditory neurons were able to meet the needs forcytological experiments in vitro. One important fact to take into account is a stable neuronal plas-ticity of anditory neuron exised in the postnatal animals under the present experimeatal conditons.This study, gathered with the previous works by author extends the material sources of innerear tissue culture and provides a reliable approach to the neurobiological researches on the periph-eral auditory system in vitro.
出处
《海军总医院学报》
1999年第3期129-133,共5页
Journal of Naval General Hospital of PLA
