摘要
目的:建立植物油类物质(火锅油、潲水油、地沟油及食用植物油)中胆固醇含量测定前处理的快捷方法,并采用超高液相-串联四极杆质谱(UPLC-MS/MS)技术定量分析。方法:采用微波萃取技术对植物油类物质进行皂化处理,再用石油醚-乙醚(1∶1)提取后,以2,2,3,4,4,6-d6胆固醇为内标,采用UPLC-MS/MS法,大气压化学电离源(APCI)正离子模式,多反应检测方式测定胆固醇浓度。结果:与传统水浴加热皂化方法前处理植物油类物质比较,微波萃取皂化前处理方法简变、快捷、高效、低污染等优点,测得胆固醇的含量几乎无显著性差别,方法回收率为98%。结论:微波萃取皂化前处理方法可用于植物油类物质中胆固醇含量测定。
Objective：To establish a microwave extraction method for improving on determination of cholesterol in vegetable oils.（hot pot oil,waste oil,illegal cooking oil and edible vegetable oil）,and conduct quantitative analysis on technology by UPLC-MS/MS.Methods： Cholesterol can be free from vegetable oils by microwave extraction method.After extracted by organic solvent,cholesterol 2,2,3,4,4,6-d6 was taken as the internal standard to detect the cholesterol level by UPLC-MS/MS,APCI＋ model and MRM mode.Results： The microwave extraction method is simpler and faster with higher efficiency and lower pollution,when compared to the traditional method.The contents of cholesterol in vegetable oils are similar on the above two methods.The recovery was 98%.Conclusion： The microwave extraction method is suitable before dispose of vegetable oils on determination of cholesterol.
出处
《中国卫生检验杂志》
CAS
2011年第7期1658-1660,共3页
Chinese Journal of Health Laboratory Technology
关键词
微波萃取技术
胆固醇
火锅油
潲水油
地沟油
食用植物油
Microwave Extraction Method
Cholesterol
Hot pot Oil
Waste Oil
Illegal cooking oil
Vegetable Oil
