摘要
目的探讨西罗莫司（SRL）和钙调磷酸酶抑制剂（CNI）对肝移植受者外周血中CD4^＋CD25^high T淋巴细胞水平的影响。方法排除肝移植远期移植肝功能异常的受者，将移植肝功能长期（超过2年）稳定的受者47例纳入研究，其中免疫抑制方案使用SRL者15例（SRL组），使用CNI（均为他克莫司）者32例（CNI组）。以同期38名健康成人志愿者作为正常对照。使用流式细胞仪检测各组受试者外周血中单个核细胞CD4、CD25及Foxp3的表达水平，比较各组间外周血中CD4^＋ CD25^high调节性T淋巴细胞（Treg细胞）的差异。结果 与正常对照组相比，CNI组外周血淋巴细胞中CD4^＋CD25^highT淋巴细胞的比例显著减少（P〈O．05），SRL组CD4^＋CD25^highT淋巴细胞的比例显著升高（P〈0．05）。SRL组、正常对照组和CNI组受试者外周血中CD4^＋CD25^highFoxp3^＋Treg细胞占CD4^＋T淋巴细胞的比例依次降低，分别为1．88％（1．56％～2．60％）、1．15％（0．57％～1．48％）和0．84％（0．46％～1．45％），3组间两两比较，差异均有统计学意义（P〈0．01或P〈0．05）。CD4^＋CD25^highT淋巴细胞表达Foxp3的阳性率超过95％，CD4^＋CD25^highT淋巴细胞表达Foxp3的阳性率低于20％，CD4^＋CD25^highT淋巴细胞不表达Foxp3。结论SRL可促进肝移植受者外周血中Treg细胞水平的升高，而CNI可降低Treg细胞的水平。
Objective To investigate the possible influence of immunosuppressive therapy, including sirolimus （SRL） and calcineurin inhibitors （CNI, tacrolimus）, on level of Treg in the liver allo-graft recipients. Methods Forty-seven liver transplant recipients with stable liver function were assessed for at least 2 years, and divided into two groups： one composed of 15 patients receiving SRL, and another, of those receiving CNI （32 patients with tacrolimus）. Thirty-eight age-matched healthy subjects （HS） were used as normal controls. We examined the expression of CD^4, CD25, and Foxp3 in peripheral blood monouclear cells. Treg of every group was analyzed using Cell Quest software. Results SRL significantly increased the prevalence of CD4^＋ CD25^high T cells, compared with HS and CNI group. The prevalence of CI）4^＋ CD25^high T cells of CNIs group was significantly lower than that of HS. The percentage of CD^4 CD25^high T cells in the total CD4＋ T cells was 1.88 % （1.56 %- 2. 611%）, 1.15 %（ 0. 57%-1.48%） and 0. 84%（0. 46 %-1.45 %） in SRL, NS and CNI group, respectively （P〈0. 01 or P〈0. 05）. Foxp3 was expressed in more than 95% of CD4^＋ CD25^high T cells and tess than 20 % of CD4^＋ CD25^high T ceils, whereas not expressed in CD4^＋ CD25^high T cells. Conclusion Different immunosuppressive therapy （SRL or CNI） might have different roles in tolerance induction in liver transplant recipients. Namely, SRL promoted the induction of allo-antigen tolerance, but CNI hampered the progression.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2011年第7期407-410,共4页
Chinese Journal of Organ Transplantation
关键词
肝移植
西罗莫司
他克莫司
T淋巴细胞
调节
Liver transplantation Sirolimus Tacrolimus T-lymphocytes regulatory
