摘要
目的研究移植后细菌感染CD4^＋ CD25^＋调节性T细胞（Treg）表达的变化。方法以DA大鼠为供体,LEW大鼠为受体,采用改良的Kamada二袖套法建立肝移植模型。术后随机分为3组,G1组术后3d腹腔生理盐水注射;G2组术后3d给予腹腔大肠杆菌注射;G3组术后常规给予免疫抑制药物CsA,3mg/（kg·d）。感染后7d处死动物,每组6个样本,检测血清ALT、TB,并采用流式细胞术检测脾脏Treg的水平,RT-PCR方法检测脾脏Foxp3和肝脏IL-10、TGF-β mRNA的表达。结果G3、G2组Treg表达水平显著升高,分别为：（16.72±0.95）%,（27.4±1.09）%,与G1（9.88±0.98）%比较差异均有统计学意义（P〈0.01）;脾脏Foxp3和肝脏IL-10、TGF-β mRNA表达水平均增加;感染组的肝功虽然较差,与排斥组相比无统计学意义,但从病理切片观察该组肝脏的急性免疫排斥反应减轻,Banff评分有统计学意义。结论大鼠肝移植术后腹腔细菌感染增加了脾脏Treg的表达,促进了免疫抑制作用的发挥,部分减轻了急性排斥反应。
Objective To investigate expression of CD4^＋ CD25 ^＋ regulatory T cells in model of abdominal cavity bacterial infection after liver transplantation in rats. Methods Orthotopic liver transplant （ OLT, DA to Lewis） models were established by modified Kamada two-cuff technique. All rats were randomly divided into 3 groups after operation. Normal saline was injected into abdominal cavity at 3rd day postoperatively in group 1, mixed Bacillus coli liquid in group 2 and CsA at 3 mg/kg per day in group 3. Six recipients were sacrificed 7 days after infection in each group, blood and graft samples were collected for detection of ALT and TB. The levels of Treg and Foxp3 in spleens and IL-10, TGF-β mRNA in livers were detected by flow cytometry and RT-PCR, respectively. Results Compared with the level of Treg for 9.88 ± 0.98 in group 1, the levels of Treg increased obviously in groups 2 and 3 （ 16.72 ± 0.95 vs 27.4 ± 1.09）, with statistical difference （ P 〈 0.01 ）, so do the expressions of Foxp3, IL-10 and TGF-β mRNA. Although the serum concentrations of AST and TB 7 d in Group 2 was higher than those in Group 1, the Banff scores of grafts in group 2 were lower than those in group 1 （ P 〈 0.05 ）. Conclusions Abdominal cavity bacterial infection after liver transplantation in rats increase expression of Treg, promote the immunosuppressive action and partially alleviate the acute rejection.
出处
《中华消化外科杂志》
CAS
CSCD
2007年第3期194-197,共4页
Chinese Journal of Digestive Surgery
