摘要
[目的]探讨骨形态发生蛋白诱导的大鼠成肌细胞应用于组织工程化人工骨修复大鼠胫骨缺损的效果。[方法]32只体重在200~250 g的雄性SD大鼠随机分成4组,每组8只。A组：聚乙烯管内植入诱导2周的成肌细胞复合透明质酸钠。B组：聚乙烯管内植入成肌细胞复合透明质酸钠。C组：聚乙烯管内植入透明质酸钠。D组：聚乙烯管内注入生理盐水。术后12周,进行X线观察、大体观察、组织学观察（HE染色）以及四环素和钙黄绿素标记。[结果]术后12周时大体观察发现,A、B组骨缺损处可见大量类骨样组织填充,未触及反常活动,C、D组可见少量胶冻样组织及类骨样组织填充,无反常活动。X线观察可见A和B组骨痂吸收,髓腔再通,C、D组有少量新骨痂,髓腔未通。组织学观察可见A和B组有大量新生骨小梁,四环素和钙黄绿素染色可见黄绿色荧光。[结论]初步结果显示经诱导的大鼠成肌细胞可作为骨组织工程的种子细胞,用于修复骨缺损。
[Objective]To investigate the effectiveness of the induced rat myoblasts used as tissue engineered bioartificial bone for bridging tibiae defects.[Method]Thirty-two Sprague Dawley adult male rats weighing 200-250 g were randomly divided into four groups of bone grafting,with 8 rats in each group.Group A： polyethylene tutes were seeded with syngeneic myoblasts induced 5 days with rhBMP-2 and sodium hyaluronate.Group B： polyethylene tutes were seeded with syngeneic myoblasts and sodium hyaluronate.Group C： polyethylene tutes were filled with sodium hyaluronate.Group D：polyethylene tutes were only filled with normal sodium.At 12 weeks,a series of examinations were performed,including morphology observation,radioactive ray,histological staining of bones,and labelling with tetracycline and calcein.[Result]At 12 weeks after operation,groups A and B had significant bone tissues than groups C and D based on morphology.For radioactive ray,groups A and B had significantly absorption of bony callus and recanalization of cavum pulpi,groups C and D had a little bony callus and significant bone defect.From histology,groups A and B had more significant new bone trabecula than groups C or D.Flavo-green fluorescence was noted.[Conclusion]Induced rat myoblasts as seeding cells for bone tissue engineering can be used to repair bone defect.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2011年第14期1201-1204,共4页
Orthopedic Journal of China
