摘要
目的观察周期性双轴力学应变对人腺样囊性癌高转移细胞株ACC-M、低转移细胞株ACC-2中E-cad和α、β、-γcat的表达以及ACC细胞与细胞外基质(ECM)粘附的影响。方法采用成组设计,分别选取频率为每分钟3次的1 000、4 000μ应变对ACC-2、ACC-M细胞分别加载,每天定点加载1次,每组加载时间分别为每次1 h、3 h和6 h,共加载2次。第3天上午8点收获细胞;将未受力学刺激的细胞作为对照组,受力学刺激的细胞作为实验组。细胞在力学刺激后行E-cad和α、β、-γcat及细胞核双重免疫荧光染色,用激光共聚焦显微镜和图像分析软件进行定量和定位观察。用MTT法测定ACC细胞和ECM的粘附率。结果对照组除ACC-2细胞E-cad低于ACC-M细胞外,ACC-2细胞α、β、-γcat表达均高于ACC-M细胞;在力学刺激下,E-cad和α、β、-γcat的表达随时间而变化。对照组ACC-2细胞和ECM的粘附率高于ACC-M细胞,但在力学刺激后,与对照组比较,ACC细胞和ECM的粘附率随时间而变化。结论在力学刺激后,ACC-2、ACC-M细胞出现E-cad/cat复合体表达的改变,同时其和ECM的粘附率也发生了改变,显示力学刺激后细胞粘附发生变化在肿瘤转移中发挥重要作用。
Objective To investigate the effects of cyclic biaxial mechanical stimulation on adhesion of salivary adenoid cystic carcinoma（SACC） extracellular matrix（ECM） and expression of E-cadherin/cateninin complex in the SACC high metastasis cell lines ACC-M, SACC low metastasis cells line ACC-2, we observed the functions of mechanical stimulation in the adhesion of SACC cell-ECM and investigate the mechanism in the adhesion of ACC. Methods Mechanical stimulation were applied to the ceils for periods of 1, 3 and 6 hours every day, lasting for 2 days. The amplitude of mechanical stimulation applied to the cells were 1 000, 4 000 μ. strain, at a frequency of 3 Hz. Unstrained cells were used as controL The expression of E complex on the cell of ACC-M, ACC-2 were studied with laser scanning confocal microscope and image analysis. SACC cell-ECM adhesion was assayed by MTT technique. Results The results showed that expressions of α、β、γ-cateninin on the cell of ACC-2 were obviously higher than that ACC-M and E-cadherin on the ceil of ACC-M were obviously higher than that ACC-2 without mechanical stimulation. Mechanical stimulation can change the expression of E-cadherin/cateninin complex on the ceil of ACC-2 and ACC-M with time. The results also showed that cell-ECM adhesion on the cell of ACC-2 were obviously higher than that of ACC-M without mechanical stimulation. Mechanical stimulation can change the cell- ECM adhesion of ACC-2 and ACC-M with time. Conclusion Mechanical stimulation can change the adhesion of the SACC ceil-ECM and expression of E-cadherin/cateninin complex of the SACC cell. We think it played an important role in metastasis of the cancer.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第1期29-32,共4页
West China Journal of Stomatology
基金
四川省科技攻关资助项目(02KW-007)
关键词
力学刺激
腺样囊性癌
细胞粘附
E—cad/cat复合体
mechanical stimulation
salivary adenoid cystic carcinoma
adhesion
E-cadherin/cateninin complex
