摘要
目的建立尿液和头发中尼古丁和可替宁的气相色谱标准检测方法。方法尿液或头发样品用NaOH溶液完全消解后用三氯甲烷萃取,经毛细管柱分离,NPD检测器检测,保留时间定性,以二苯胺作为内标物进行定量。结果尿液中尼古丁和可替宁的检测范围分别为0.017～5μg/ml和0.024～5μg/ml,重复测定相对标准偏差(RSD)分别为1.7%～2.1%和1.5%～2.8%,加标回收率分别为102%～104%和90%～106%,回归方程分别为y=2.508x+0.072,r=0.999 2和y=1.797x+0.050 9,r=0.999 6;头发中尼古丁的检测范围为0.017～4μg/ml,重复测定相对标准偏差(RSD)为0.87%～2.5%,加标回收率92.5%～96.1%,回归方程为y=2.456x-0.054 6,r=0.999 8。本方法测定尼古丁和可替宁时,在进样1μl条件下,检出限分别为0.005μg/ml和0.007μg/ml,测定下限分别为0.017和0.024μg/ml。结论本方法适合测定主动吸烟和被动吸烟者体内的尼古丁和可替宁含量,评估暴露者的烟草暴露剂量和程度。
Objective To establish a gas chromatography standard method for the determination of nicotine and cotinine in urine and hair. Methods The urine and hair samples were digested with NaOH, extracted with traehloromethane, separated by capillary column and determined with NPD detector, diphenylamine as the internal standard substance. Results The samples of nicotine and eotinine were injected at 1 μl respectively, and the limits of detection were 0.005 μg/ml and 0.007 μg/ml, and limits of quantification were 0.017 μg/ml and 0.024 μg/ml respectively. The linear ranges of nicotine and cotinine in urine were 0.017-5 μg/ml and 0.024-5 μg/ml, the relative standard deviations（RSD） of determination were 1.7%- 2.1% and 1.5%-2.8%, the rates of recovery of spiked samples were in the range of 102%-104% and 90%-106%, and the regression equations were y=2.508x＋0.072, r=0.999 2 and y=1.797x＋0.050 9, r=0.999 6. The linear range of nicotine in hair was 0.017-4 μg/ml, the relative standard deviations（RSD） of determinations were 0.87%-2.5%, the rates of recovery of spiked samples was in the range of 92.5%-96.1%, and the regression equation was y=2.456x-0.054 6, r=0.999 8. Conclusion This method is applicable to the determination of nicotine and cotinine in active smoking and passive smoking people and the evaluation of tobacco exposure level.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第7期626-628,共3页
Journal of Environment and Health
基金
国家"十一五"科技部支撑计划(2008BAI56B07)
