摘要
目的研究M受体不同亚型对孤啡肽(N/OFQ)抑制大鼠顶叶皮质神经元延迟整流钾电流(IK)作用的影响。方法采用全细胞膜片钳技术,应用M受体不同亚型阻断剂,观察M受体亚型在孤啡肽抑制大鼠顶叶皮质神经元IK中的作用。结果 (1)N/OFQ对大鼠顶叶皮质神经元IK有抑制作用,抑制率为30.8%±5.6%(P<0.01)。(2)M1受体拮抗剂哌伦西平(PIR)可减弱N/OFQ对IK的抑制作用,在指令电压为+60 mV时,PIR+N/OFQ组的IK幅度下降了15.5%±2.5%,与单独给予N/OFQ所引起的IK抑制效应相比有显著差异(P<0.05)。(3)M3受体拮抗剂4DAMP拮抗N/OFQ对IK的抑制作用不明显。结论 M1受体参与N/OFQ对大鼠顶叶皮质神经元IK的抑制作用。
Objective To study the effect of M1 and M3 receptors＇ antagonists on the inhibition of delayed rectifier potassium current（IK） by nociceptin/orphanin FQ（N/OFQ） in dissociated rat cerebral parietal cortical neurons.Methods Using whole-cell patch-clamp technique,recording effects of different subtypes of M receptor on the inhibition of IK by N/OFQ in rat cerebral cortical neurons.Results（1）N/OFQ inhibited IK in acutely dissociated rat cerebral parietal cortical neurons,and the inhibition rate was 30.8%±5.6%（P0.01）;（2）PIR M1 receptor antagonist antagonized the inhibitory effect of N/OFQ on IK and the drop of currents varied from the control value of 30.8%±5.6% to 15.5%±2.5%（P0.05）;（3） M3 receptor antagonist 4DAMP had no obvious effects on the inhibitory effect of N/OFQ on IK.Conclusions M1-receptor is involved in the inhibition of N/OFQ on IK in acutely dissociated rat cerebral parietal cortical neurons.
出处
《基础医学与临床》
CSCD
北大核心
2011年第8期853-857,共5页
Basic and Clinical Medicine
基金
国家自然科学基金
青年基金(30900489)
关键词
孤啡肽
M受体亚型阻断剂
顶叶皮质
延迟整流钾电流
全细胞膜片钳技术
nociceptin/orphanin FQ
M receptor subtype
pariental cortex
delayed rectifier potassium current
whole-cell path-clamp technique
