摘要
目的:探讨CD4+CD25+CD127low/-调节性T细胞和转化生长因子β(transforming growth factor-β,TGF-β)在初发活动性类风湿关节炎(rheumatoid arthritis,RA)患者外周血中的表达及其在RA发病机制中作用。方法:采用流式细胞术检测40例初发活动性RA患者(RA组)和35例正常对照者(对照组)外周血CD4+CD25+CD127low/-调节性T细胞阳性率,采用酶联免疫吸附试验检测其血清中TGF-β水平,并进行统计分析。结果:RA组CD4+CD25+ T细胞百分率明显低于对照组(P<0.05);CD4+CD25+CD127low/-调节性T细胞百分率低于对照组,但差异无统计学意义(P>0.05)。2组CD4+CD25+T细胞与CD4+CD25+CD127low/-调节性T细胞百分率比较,差异均有统计学意义(P<0.05)。同组CD4+CD25+T细胞与CD4+CD25+CD127low/-调节性T细胞表达水平均有相关性(P<0.05)。RA组TGF-β水平明显高于对照组(P<0.01);2组CD4+CD25+T细胞和CD4+CD25+CD127low/- T细胞水平与其血清TGF-β表达水平无相关性(P>0.05);三者表达水平均与RA活动指标无相关性(P>0.05)。结论:RA患者CD4+CD25+CD127low/-调节性T细胞数量减少可能与RA的细胞免疫功能紊乱有关。
Objective To investigate the expressions of CD4＋CD25＋CD127low/-regulatory T cells and transforming growth factor-β in the peripheral blood of patients with primary and active rheumatoid arthritis and to explore its mechanism in the pathogenesis of rheumatoid arthritis.Methods The rates of CD4＋CD25＋CD127low/-regulatory T cells in the peripheral blood of 40 primary and active rheumatoid arthritis patients and 35 normal controls were evaluated with flow cytometry.The level of transforming growth factor-β in blood serum was detected with ELISA.All the data were analyzed.Results The positive rate of CD4＋CD25＋ T cells in rheumatoid arthritis was significantly lower than that in normal controls（P0.05）.The positive rate of CD4＋CD25＋CD127low/-T cells in rheumatoid arthritis was lower than that in normal controls,but there was no significant difference（P0.05）.There were significant differences between the percentage of CD4＋CD25＋ T cells and the percentage of CD4＋CD25＋CD127low/-T cells in two groups（P0.05）.The level of CD4＋CD25＋ T cells was correlated with the level of CD4＋CD25＋CD127low/-T cells in both groups（P0.05）.The level of transforming growth factor-β in rheumatoid arthritis was higher than that in control group（P0.01）. Transforming growth factor-β was not correlated with the level of CD4＋CD25＋CD127low/-regulatory T cells or CD4＋CD25＋ T cells in two groups（P0.05）.The levels of CD4＋CD25＋CD127low/-regulatory T cells,CD4＋CD25＋ T cells and transforming growth factor-β were not correlated with erythrocyte sedimentation rate,C-reactive protein,anticyclic citrullinated peptide antibody,antikeratin antibody,or rheumatoid factor level（P0.05）.Conclusion The decrease of CD4＋CD25＋CD127low/-regulatory T cells may be correlated with cellular immunity disorder in rheumatoid arthritis.The mechanism of transforming growth factor-β is complex in rheumatoid arthritis.
出处
《中华实用诊断与治疗杂志》
2011年第7期680-683,共4页
Journal of Chinese Practical Diagnosis and Therapy
