摘要
目的研究疏血通注射液对大鼠背部随意型皮瓣存活的影响。方法采用改良的大鼠McFarlane皮瓣制作方法,将Sprague Dawley大鼠随机分为疏血通实验组和生理盐水对照组,实验组每只每天腹腔注射疏血通注射液1.5mL/kg,对照组每只每天腹腔注射生理盐水1.5mL/kg。术后第7天处死大鼠分别进行皮瓣存活面积比的检测,并取皮瓣近、中、远区组织切片光镜下观察,免疫组化法检测血管内皮生长因子(VEGF)的表达。结果术后7d,实验组皮瓣的存活面积比为(72.52±2.23)%,对照组皮瓣的存活面积比为(50.36±2.37)%,实验组存活面积比显著高于对照组(P<0.01)。实验组中区皮瓣的组织水肿、炎症细胞浸润情况明显比对照组减轻,并且出现大量的新生血管,中区新生血管密度(27.42±4.21)个/mm2,与对照组(17.45±5.43)个/mm2差异有统计学意义(P<0.05)。实验组VEGF表达量为4731.24±448.99,对照组为2466.01±801.67,差异有统计学意义(P<0.01)。结论疏血通注射液可能通过增加VEGF表达等途径,促进皮瓣新生血管增生,改善皮瓣血供,减轻炎症反应,从而改善大鼠随意型皮瓣的存活。
Objective To investigate the effects of Shuxuetong on murine random pattern skin flaps. Methods McFarlane flap （3 cm × 9 cm） model was established on the rat dorsum. A total of 24 Sprague Dawley rats were randomly divided into a control group and an experimental group. ShtLxuetong injection （1.5 mL/kg） was administered intraperitoneally in the experimental group immediately after the operation and an equal volume of saline to the control group. The viability of the tissue was measured at day 7 and the tissue samples from proximal ,middle,and distal portions were sectioned with HE staining for histological analysis. Vascular endothelial growth factor （VEGF） was detected by immunohistochemistry in the middle portion. Results On postoperative day 7, the percentage of the surviving area of the flap in the experimental group was significantly higher than that of the control group [ （72.52±2.23） % vs （50.36±2.37） %, P 〈 0.05 ]. In the middle portion, edema and infiltration of neutrophils observed in tissue sections in the experimental group were reduced than those of the control group, and new blood vessels increased in the experimental group （P 〈 0.05 ）. A significant difference was noted in the expression of VEGF between the experimental and control groups （4731.24±448.99 vs 2466.01±801.67, P 〈 0.01 ）. Conclusion Shuxuetong may enhance neovascularization and increase skin flap blood supply by elevating the expression of VEGF in skin flaps, and eventually promote the survival of the flaps.
出处
《中华移植杂志（电子版）》
CAS
2011年第1期18-21,共4页
Chinese Journal of Transplantation(Electronic Edition)
基金
浙江省中医药科技计划项目资助课题(2009CA076)
关键词
疏血通注射液
随意型皮瓣
大鼠
存活
新生血管
血管内皮生长因子
Shuxuetong injection
Random pattern skin flap
Rats
Survival
Neovascularization
Vascular endothelial growth factor
