摘要
为了分析新疆多浪羊的IFN-γ基因,根据GenBank中NM_001009803的mRNA序列设计引物,以新疆多浪羊淋巴细胞的总RNA为模板,用RT-PCR扩增多浪羊IFN-γ基因序列。测序结果表明I,FN-γ基因序列包含了一个582 bp的开放阅读框,通过GenBank中的Blastn序列比较分析,多浪羊的IFN-γ基因与普通绵羊的同源性高达99.82%,为多浪羊IFN-γ的功能研究及多浪羊免疫应答基因的筛选奠定基础。
According to mRNA sequence（NO.NM_001009803） from GenBank,one pair of primers was designed.The IFN-γ gene was amplified from total RNA template of duolang sheep＇s lymphocytes by RT-PCR.The fragment was obtained and its length was about 564bp and then combinated with pMD18-T vector.Then recombinant vector was transformed into DH5α.The final recombination strain mixed with 30% glycerol to determinat nucleotide sequence.The result showed that the cDNA sequence of IFN-γ contains one ORF of 582 bp.The comparison analysis of the fragment sequence with other ovis through blast in GenBank.The homologies of the nucleotide sequence with ovis were 99.82%.This research provides the foundation on the function of duolang＇s IFN-γ and it laies the foundation for Screening of the Immune Response Related Genes from duolang sheep.
出处
《塔里木大学学报》
2011年第2期19-23,共5页
Journal of Tarim University
基金
国家自然科学基金(30960277)
