摘要
有机聚合物整体柱具有制备简单、易于改性和通透性好的优点,不仅可作为微柱色谱分离分析的固定相,而且可用作样品纯化富集的载体,广泛应用于基体复杂的生化样品分析领域。该文综述了近年来有机聚合物整体柱的研究进展,重点介绍了其制备方法、改性技术及在生化样品预处理领域的研究动态。针对其制备方法,从制备过程、制备原料(单体、交联剂、引发剂及致孔剂)和引发方式等方面进行概述;并根据改性方式将有机聚合物整体柱改性技术分为聚合物整体柱表面的衍生改性、纳米材料与有机整体柱结合的改性技术及有机-无机杂化整体柱改性3个方面;同时将其在生化样品预处理尤其是在毛细管电色谱(CEC)、微柱液相色谱(μ-HPLC)、微分析系统(μ-TAS)中的样品预处理研究进展进行了总结。结合有机聚合物整体柱目前的研究现状,对其应用前景和发展方向进行了展望。
As a kind of chromatographic columns developed rapidly in recent years,the monolithic columns have been widely applied in biological sample analysis for their high resolution,low-cost preparation,long service life,good stability and other advantages.The recent development of organic polymer monolithic columns was reviewed.The preparation process,raw materials for preparation（monomer,crosslinker,initiator and porogen） and initiation methods were outlined.In addition,the modified organic polymer monolithic columns made up of organic polymer monolith with the derived surface,monolithic column combining the nano-materials with organic polymer monolith and organic-inorganic hybrid monolith were discussed according to the ways of modification technology.The application of organic polymer monolithic columns in the pretreatment of biological sample were summarized,especially in capillary electrochromatography（CEC）,microcolumn liquid chromatography（μ-HPLC） and micro-analysis system（μ-TAS）.Finally,the trends and future perspectives of organic polymer monolithic columns were outlined.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第7期825-834,共10页
Journal of Instrumental Analysis
基金
重庆市科委科技计划攻关项目(CSTC
2010AC5050)
国家科技部科技人员服务企业行动项目(2009GJF10037)
重庆大学研究生科技创新基金项目(CDJXS10-22-11-39)
重庆大学211工程三期创新人才培养计划建设项目(S-09103)
国家大学生创新性实验计划资助项目(091061141)
关键词
有机聚合物整体柱
改性
应用
进展
综述
organic polymer monolithic columns
modification
application
development
review
