摘要
采用甲基丙烯酸丁酯(BMA)为单体、乙二醇二甲基丙烯酸酯(EDMA)为交联剂,合成了聚甲基丙烯酸丁酯-乙二醇二甲基丙烯酸酯(poly(BMA-co-EDMA))整体柱,并将其作为固相萃取整体柱与毛细管电泳联用,建立了桑叶中芦丁、绿原酸、槲皮素等多酚物质的分离检测方法。考察了固相萃取条件如淋洗液种类、淋洗液流速和体积、洗脱液种类对萃取效率的影响,并采用毛细管电泳进行分离分析。结果显示,芦丁、绿原酸、槲皮素均在10~160μg/mL范围内线性关系良好,相关系数分别为0.9955、0.9985、0.9995,检出限分别为4.65、1.12、3.49μg/mL,相对标准偏差分别为2.4%、0.98%、2.8%,平均回收率分别为94.7%、98.9%、102%。该固相萃取柱能有效富集和纯化桑叶中3种多酚物质。方法简便、快速、灵敏,适用于桑叶中3种多酚类物质的分离分析。
A capillary electrophoresis with monolithic column solid phase extraction was established for the separation and detection of polyphenols such as rutin,chlorogenic acid and quercetin in mulberry leaves.The polybutyl methacrylate-ethylene glycol dimethacrylate(poly(BMA-co-EDMA)) monolithic column was synthesized by using butyl methacrylate(BMA) as monomer and ethylene glycol dimethacrylate(EDMA) as crosslinker.Effects of solid phase extraction conditions such as leacheate type,flow rate and volume of leacheate,eluent type on extraction efficiency were investigated,and the separation and analysis of three polyphenols were performed by capillary electrophoresis.Under the optimized conditions,there were good linear relationships for rutin,chlorogenic acid and quercetin in the range of 10-160 μg/mL with correlation coefficients of 0.995 5,0.998 5 and 0.999 5,respectively.The detection limits for rutin,chlorogenic acid and quercetin were 4.65,1.12,3.49 μg/mL,respectively.The average recoveries for three analytes were 94.7 %,98.9 % and 102 %,with relative standard deviations of 2.4 %,0.98 % and 2.8 %,respectively.Results showed that the solid phase extraction column could be used for the effective enrichment and purification of three polyphenols in mulberry leaves.The method is simple,rapid and sensitive,and is suitable for the separation and analysis of three polyphenols in mulberry leaves.
作者
李晓慧
徐志辉
李利军
程昊
冯军
LI Xiao-hui;XU Zhi-hui;LI Li-jun;CHENG Hao;FENG Jun(Guangxi Key Laboratory of Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Medical School,Guangxi University of Science and Technology,Liuzhou545005,China;Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry,Nanning530004,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2019年第7期805-810,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金地区科学基金项目(31560466,81860633)
广西自然科学基金面上项目(2016GXNSFAA380108)
广西高等学校高水平创新团队及卓越学者计划资助项目(桂教人[2014]7号)
关键词
整体柱
固相萃取
桑叶多酚
monolithic column
solid phase extraction
mulberry leaf polyphenols
