摘要
利用RT-PCR技术从甜荞中克隆得到查耳酮合酶（CHS）的cDNA开放阅读框（ORF）序列,命名为FeChs,NCBI登录号为GU172166.1。该序列长1 179 bp,编码392个氨基酸,与其它植物CHS基因的同源性为78%~92%,其推导的氨基酸序列含有CHS高度保守的活性位点及CHS的标签序列GFGPG。
The cDNA ORF sequence of chalcone synthase gene（FeChs）was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants＇CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG.
出处
《广西植物》
CAS
CSCD
北大核心
2011年第3期383-387,共5页
Guihaia
基金
四川省科技攻关项目(2006Z08-012)~~
关键词
甜荞
查耳酮合成酶基因
克隆
序列分析
Fagopyrum esculentum
chalcone synthase gene
cloning
sequence analysis
