摘要
目的:明确杜仲叶和果实中的总黄酮和总多糖的含量,为杜仲叶和果实的最佳采收期的确定以及开发提供科学的依据。方法:不同采收期杜仲叶和果实,分别用70%乙醇加热回流提取3次,紫外分光光度法(506 nm)对其总黄酮的含量进行测定,进行系统的分析统计,得出总黄酮含量最高的月份。不同采收期杜仲叶和果实,分别用水加热回流提取3次,用苯酚-硫酸显色,用紫外分光光度法(481 nm)对其总多糖的含量进行测定,进行系统的分析统计,得出总多糖含量最高的月份。结果:紫外分光度法测得不同季节的杜仲叶和果实中总黄酮和总多糖的含量有显著差异,叶和果实中总黄酮含量以4月份为最高。叶和果实中总多糖含量均以3月份为最高,但是果实中总多糖的含量在6月份会达到最低,7月份又有显著回升;而叶的这个过程不明显,5月份后总多糖含量一直缓慢下降。结论:杜仲叶和果实中的总黄酮和总多糖的含量均比较高,其最佳采收期均为4月份。
Objective：To make clear the peak content of total flavonoids and total polysaccharide in the leaf and fruit of Eucommia ulmoides,so as to provide evidence for the optimal harvest of the fruit of E.ulmoides.Method：Total flavonoids extracted with 70% ethanol by reflux from leaves and fruits of E.ulmoides in different harvest periods were determined by UV spectroscopy at 506 nm.Total polysaccharide from leaves and fruits of E.ulmoides in different harvest periods were determined by phenol-sulfuric acid method and measured by ultraviolet spectrophotometer at 481 nm with glucose as a reference substance.Result：The flavonoids content in leaves and fruits of E.ulmoides during different harvest periods were significant different by UV spectroscopy.The highest flavonoids content of leaves and fruits during different harvest periods of E.ulmoides was found in April,and then declined until July and a little rise again.The content of flavonoids stabilized after September.There were significant differences about total polysaccharide in leaves and fruit of E.ulmoides in different seasons.The total polysaccharide content of the leaves and fruits of Eucommia in different harvests were highest in March.The content of polysaccharide of the Eucommia leaf gradually decreased after May.And then,total polysaccharide content of Eucommia fruits would stabilize from August to November.Conclusion：It can be obtained from this experiment that the best harvest period of the leaves and fruits of Eucommia is in April.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期111-114,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省唐山市科技局科研项目(09130223b)
关键词
杜仲叶
杜仲果实
总黄酮
总多糖
最佳采收期
Eucommia ulmoides leaf
Eucommia ulmoides fruit
total flavonoids
total polysaccharide
different harvest periods
