摘要
目的:优化金莲花总黄酮的超声提取工艺。方法:以总黄酮得率为指标,采用响应面设计方法对影响总黄酮得率的乙醇体积分数、提取时间及料液比进行优化。结果:最佳工艺条件为采用49%乙醇,料液比1∶43,在50℃下超声提取22min,在此最佳条件下,总黄酮得率为12.02 mg.g-1,实验结果与模型预测值相符。结论:利用超声方法提取金莲花总黄酮工艺稳定可靠。
Objective：To optimize the ultrasound-assisted extraction technology of total flavonoids from Trollius chinensis Bunge.Method： The factors such as ethanol concentration,extraction time and liquid-material ratio were optimized by response surface methodology.Result： The highest extraction rate of total flavonoids by ultrasound-assisted extraction was 12.02 mg·g-1 using ethanol concentration 49 percent,liquid-material ratio of 1∶43,extraction temperature at 50 ℃,and extracted for 22 min,which was agreed with model predictions.Conclusion： The extraction process by ultrasound-assisted was stable and reliable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期35-38,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏大学大学生科研立项项目(Y09A098)
关键词
金莲花
总黄酮
超声提取
响应面设计
Trollius chinensis
total flavonoids
ultrasonic-assisted extraction
response surface methodology
