摘要
目的:通过研究黄芪当归配伍对气道变应性炎症模型大鼠鼻黏膜及肺组织GATA-3、T-bet表达的影响,探讨黄芪当归配伍治疗气道变应性炎症的作用机制。方法:采用卵清蛋白(OVA)喷雾的方法致敏造成大鼠气道变应性炎症模型,分别用黄芪、当归、黄芪当归配伍灌胃给药治疗。用免疫组化法测定鼻黏膜中及肺组织中GATA-3、T-bet的表达。结果:模型组鼻黏膜及肺组织GATA-3表达增加,而T-bet表达降低。当归组可降低肺组织GATA-3表达,增加鼻黏膜T-bet的表达;黄芪组可降低肺组织GATA-3表达,升高鼻黏膜及肺组织T-bet表达;芪归组可降低鼻黏膜及肺组织GATA-3表达,升高鼻黏膜和肺组织T-bet表达。各给药组均可降低鼻黏膜及肺组织中GATA-3/T-bet比值。结论:黄芪当归配伍可能通过对GATA-3/T-bet的影响,调节气道变应性炎症中Th1/Th2细胞因子平衡,从而对气道变应性炎症有抑制作用。
Objective：To observe the Influence of HuangQi and DangGui on levels of GATA-3 and T-bet in nasal mucosa and lung tissue of airway allergic inflammation rats,in order to partly show the mechanism of HuangQi and DangGui treating airway allergic inflammation.Methods：Using the model of airway allergic inflammation rats caused by OVA,we observed the effect of HuangQi and DangGui on expression of GATA-3 and T-bet in nasal mucosa and lung tissue by immunohistochemical staining method.Results：The expression of GATA-3 in nasal mucosa and lung tissue were significantly higher than those in the control group.The expression of T-bet in nasal mucosa and lung tissue were significantly lower than those in the control group.DangGui can depress the expression of GATA-3 in lung tissue and increase the expression of T-bet in nasal mucosa.HuangQi can depress the expression of GATA-3 in lung tissue,and increase the expression of T-bet in nasal mucosa and lung tissue.Compatibility of HuangQi and DangGui can depress the expression of GATA-3 in nasal mucosa and lung tissue,and increase the expression of T-bet in nasal mucosa and lung tissue.Conclusion：Compatibility of HuangQi and DangGui may act on GATA-3 and T-bet,to adjust the balance of Th1 and Th2 on airway allergic inflammation.
出处
《中华中医药学刊》
CAS
2011年第7期1661-1663,共3页
Chinese Archives of Traditional Chinese Medicine
