摘要
目的探讨联合检测上皮细胞抗原(epithelial antigen,EA)、E-cadherin、癌胚抗原(corcinoembryonic antigen,CEA)、细胞角蛋白5/6(cytokeratin5/6,CK5/6)、Calretinin和甲状腺转汞因子(thyraid transcription factor-1,TTF-1)在胸腔积液鉴别诊断中的价值。方法经临床及影像学资料和/或组织学检查证实的34例转移性腺癌、29例反应性间皮增生的胸腔积液标本,每例均做细胞块行免疫细胞化学染色。结果 EA、E-cadherin、CEA及TTF-1在转移性腺癌的阳性表达率为91.2%、88.2.4%、85.3%、91.2%,特异性为100%、100%、96.6%、96.6%;而Calretinin和CK5/6诊断间皮源性细胞的阳性表达率为100%、93.1%,特异性为97.1%、94.1%。结论 EA、E-cadherin、CEA、CK5/6、Calretinin、TTF-1是适用于胸腔积液转移性腺癌和间皮源性细胞鉴别有价值的一组抗体。
Objective To evaluate the value of combining detection of epithelial antigen（EA）,E-cadherin,carcinoembryonic antigen（CEA）,thyroid transcription factor-1（TTF-1）,calretinin,and cytokeratin 5/6（CK5/6）in differential diagnosis of pleural effusion.Methods Pleural effusion specimens of 34 cases of metastatic adenocarcinoma and 29 cases of reactive mesothelium hyperplasia were prepared cell blocks.Immunocytochemical staining was performed on cell block sections.Results In diagnosing metastatic adenocarcinoma,the sensitivities of EA,E-cadherin,CEA and TTF-1 were 91.2%,88.2.4%,85.3% and 91.2%,the specificities were 100%,100%,96.6% and 96.6%.The sensitivity of calretinin and CK5/6 for mesothelial cells was 100%,93.1%,and the specificity was 97.1%,94.1%.Conclusion The combination of EA,E-cadherin,CEA,TTF-1,calretinin and CK5/6 is a very useful antibody in cytological diferential diagnosis of metastatic adenocarcinoma and mesothelial cells in pleural effusion.
出处
《实用医院临床杂志》
2011年第4期91-93,共3页
Practical Journal of Clinical Medicine
关键词
胸腔积液
腺癌
反应性间皮增生
免疫细胞化学
鉴别诊断
Pleural effusion
Adenocarcinoma
Reactive mesothelial hyperplasia
Immunocytochemistry
Differential diagnosis
