摘要
苯巴比妥钠诱导增殖的小鼠肝脏加热提取物５ ～２００ｍｇ／Ｌ，明显刺激人Ｌ０２ 肝细胞系３ Ｈ－ ＴｄＲ掺入，抑制５ － Ｆｕ 对人Ｌ０２ 肝细胞的杀伤作用； 相同剂量的非加热提取物抑制人Ｌ０２ 肝细胞生长。苯巴比妥钠５ ～２０ｍｇ／Ｌ，对人Ｌ０２ 细胞３ Ｈ－ ＴｄＲ 掺入及５ － Ｆｕ 杀伤作用无影响。经胰蛋白酶预处理后， 小鼠增殖肝加热提取物活性消失。这说明苯巴比妥钠本身对人Ｌ０２ 肝细胞不是一种丝裂原性药物， 其诱导小鼠肝脏增殖作用与肝脏局部体液因素有关， 该因素为蛋白或肽类物质。
The heated extract from mouse hyperplastic liver induced by phenobarbital sodium (5～200mg/L) significantly stimulated the incorporation of 3H-TdR in human L 02 liver cell line, and inhibited the killing of 5-Fu on the cell line. The similar dose of the unheated extract inhibited the growth of the L 02 cell. Phenobarbital sodium 5～20mg/L did not affect the incorporation of 3H-TdR in the L 02 cell line and the killing action of 5-Fu on it. The activity of the heated extract was eliminated by the pretreatment with trypsin. The results showed that phenobarbital itself was not a mitogenic drug in the L 02 cell culture and the induction of hepatic proliferation by phenobarbital was related to local humoral factor in mouse liver, which was an intrinsic protein or peptide substance.
出处
《标记免疫分析与临床》
CAS
1999年第4期211-214,共4页
Labeled Immunoassays and Clinical Medicine
