摘要
目的:探讨乙醇对骨髓间充质干细胞(BMSCs)中神经生长因子(NGF)、成纤维细胞生长因子(FGF)、降钙素相关基因肽受体(CGRPR)、血管活性肠肽受体(VIPR)、P物质受体(SPR)及过氧化物酶体增殖子活化受体-γ(PPARγ)表达的影响。方法:收集第3代兔BMSCs,随机分为2组,实验组加入终浓度为0.09mol/L的乙醇,对照组正常培养,于培养的第4、7天,采用荧光定量PCR测定2组细胞中上述基因mRNA的表达。结果:第4和7天,实验组BMSCs中FGF、CGRPR、VIPR、NGF和SPR的mRNA表达均较对照组降低(第4天:t=5.327、34.773、12.036、28.660和22.584,P均<0.01;第7天:t=4.664、44.965、8.461、12.809和35.163,P均<0.01),PPARγmRNA表达增高(t=31.107和36.775,P均<0.001)。结论:乙醇可以抑制兔BMSCs中成骨相关神经递质或其受体的表达,促进PPARγ的表达,从而参与酒精性股骨头坏死的发病。
Aim： To investigate the effect of alcohol on the mRNA expressions of fibrocyte growth factor（FGF）,calcitontin gene-related peptide receptor（CGRPR）,vasoactive intestinal peptide receptor（VIPR）,nerve growth factor（NGF）,substance P receptor（SPR） and peroxisom proliferator-activated receptor（PPAR）in bone marrow mesenchymal stem cells（BMSCs） of rabbits.Methods：BMSCs of rabbits were seperated and divided into two groups randomly.The cells of the experimental group were treated with 0.09 mol/L ethanol,and the cells of the control group were treated without ethanol.The mRNA expressions of neurotransmitters mentioned above in the cells were investigated by RT-PCR at Day 4 and 7.Results：The mRNA expressions of FGF,CGRPR,VIPR,NGF and SPR of the experimental group were significantly lower than those of the control group（Day 4：t=5.327,34.773,12.036,28.660 and 22.584,P0.01;Day 7：t=4.664,44.965,8.461,12.809 and 35.163,P0.01）,but that of PPARγ mRNA increased （t=31.107 and 36.775,P0.001）.Conclusion：Alcohol could inhibit the expressions of neurotransmitters mentioned above in BMSCs,which is involved in pathogenesis of the alcohol-induced osteonecrosis of femoral head.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第3期357-360,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30672128
关键词
乙醇
股骨头坏死
骨髓间充质干细胞
神经递质
兔
alcohol
necrosis of femoral head
bone marrow mesenchymal stem cell
neurotransmitter
rabbit
