摘要
目的:研究模拟高原低压低氧环境下大鼠血清和胃黏膜中Ghrelin表达的变化,探讨高原环境对消化系统功能的影响.方法:30只Sprague-Dawley大鼠随机均分为海平面对照组、3000和5000m模型组;采用小动物低压舱模拟不同海拔高度,舱内停留时间为24h.应用ELISA方法测定各组动物动血清中Ghrelin的浓度;用免疫组织化学方法观察大鼠胃黏膜组织中Ghrelin的定位与表达,并比较各组动物间胃黏膜Ghrelin免疫反应阳性细胞密度的差异.结果:与海平面对照组相比,3000m和5000m模型组大鼠血清Ghrelin浓度(μg/L)明显低于地面对照组(7.45±1.33,6.87±1.32vs9.31±1.65;P<0.05或0.01).Ghrelin免疫反应阳性细胞分布于大鼠胃腺X/A细胞和内分泌细胞、阳性物质定位于细胞质.与海平面对照组相比,3000m和5000m模型组大鼠胃黏膜Ghre-lin阳性细胞密度显著降低(103.9±9.55,97.7±11.22vs119.9±11.96;P<0.01或0.001).结论:模拟高原的低压低氧环境可致大鼠血清Ghrelin水平下降,胃黏膜Ghrelin分泌减少,Ghrelin可能参与模拟低压低氧环境中机体消化功能紊乱的调节.
AIM: To investigate the effect of simulated high-altitude hypobaric hypoxia on serum and gastric levels of ghrelin in rats and to provide experimental evidence regarding the effect of high-altitude hypobaric hypoxia environment on digestive system function. METHODS: Thirty Sprague-Dawley rats were randomly divided into three groups and ex- posed to ground level (control group) and asimulated altitude of 3 000 m and 5 000 m for 24 hours, respectively. ELISA was used to detect serum ghrelin levels, while the localization and expression of ghrelin in rat gastric mucosa were detected by immunohistochemistry. The density of gastric mucosal ghrelin immunoreactive cells in gastric mucosa was compared among each group. RESULTS: Serum ghrelin concentrations in rats exposed to a simulated altitude of 3000 m and 5000 m were signif icantly lower than that in control rats (7.45 μg/L ± 1.33 μg/L, 6.87 μg/L ± 1.32 μg/L vs 9.31 μg/L ± 1.65 μg/L; P 0.05, 0.01). Ghrelin immunoreactive cells were distributed in the X/A-like cells and endocrine cells of the fundic gastric glands, and the positive staining signals were mainly located in the cytoplasm. The integrated optical density of ghrelin immunoreactive cells in rats exposed to a simulated altitude of 3000 m and 5000 m was signif icantly lower than that in control rats (103.9 ± 9.55, 97.7 ± 11.22 vs 119.9 ± 11.96; both P 0.01). CONCLUSION: Simulated acute hypobaric hypoxia reduces serum and gastric levels of ghrelin in rats, suggesting that ghrelin might be involved in the regulation of digestive system dysfunction in hypobaric hypoxia environment.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第16期1726-1730,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30872319
西藏民族学院科研基金资助项目
No.06MYY15~~