摘要
目的通过检测Ki-67在宫颈上皮内瘤变(CIN)及宫颈鳞癌中的表达与高危型HPV感染的相关性研究,探讨Ki-67能否成为CIN预后指标之一。方法对147例CIN病变上皮、29例宫颈鳞癌上皮和30例正常宫颈上皮行高危型HPV检测,并用免疫组化染色检测Ki-67表达情况。结果①随着病变的加重,Ki-67的平均灰度值逐渐提高(CINⅡ组除外)。宫颈鳞癌组的Ki-67的平均灰度值与其他各组比较有统计学差异(P<0.05),CINⅠ、Ⅲ组与对照组比较有统计学差异(P<0.05),CINⅢ组与CINⅡ组比较有统计学差异(P<0.05)。②高危型HPV在对照组、CINⅠ-Ⅲ组、宫颈鳞癌组中表达阳性率分别为0,52.63%,58.82%,82.05%,100%,除CINⅡ组与CINⅠ组比较无统计学差异(P>0.05),其余各组间高危型HPV阳性率比较均有统计学差异(P<0.05)。③Ki-67与高危型HPV感染呈正相关(r=0.228,P<0.05)。结论 Ki-67可能成为高危型HPV感染患者的CIN预后指标之一。
Objective To investigate the expression of Ki-67 in cervical intraepithelial neoplasia（CIN） and cervical squamous cell carcinoma and its relationship with HPV infection,and to explore the role of Ki-67 in evaluation of the prognosis of CIN. Methods Ki-67 protein expression was detected in 147 cases of CIN,29 cases of cervical squamous cell carcinoma（CSC） and 30 cases of normal cervical epithelium by immunohistochemistry.The high-risk HPV infection was detected in all cases. Results The average gray scale of Ki-67 gradually increased（except in CIN Ⅱ group）.The average gray scale of Ki-67 in cervical squamous cell carcinoma group was significantly higher than in the other groups（P〈0.05）.The average gray scale of Ki-67 was significantly higher in CINⅠ,Ⅲ group than in control group（P〈0.05）,and also higher in CINⅢ group than in CINⅡ group（P〈0.05）.The positive rates of high-risk HPV were 0 in normal controls,52.63% in CIN Ⅰ,58.82% in CIN Ⅱ,82.05% in CIN Ⅲ and 100% in CSC.The positive rate of high-risk HPV was statistical different between 5 groups except between CIN Ⅱ group and CIN Ⅰgroup.There was a positive correlation between Ki-67 and HPV infection（r=0.228,P〈0.05）. Conclusion Ki-67 can be used as a predictive index for prognosis of CIN with HPV infection.
出处
《山西医科大学学报》
CAS
2011年第6期488-490,共3页
Journal of Shanxi Medical University
关键词
宫颈上皮内瘤变
宫颈鳞癌
人乳头瘤病毒
KI-67
cervical intraepithelial neoplasia； cervical squamous cell carcinoma； human pappilloma virus； Ki-67
