摘要
以福伊A、谷丰A和全丰A等抗稻瘟病水稻不育系选育系谱中的谷农13、天谷B、福伊B、谷丰B、全丰B为母本,以CO39为轮回亲本的6个近等基因系和感病对照丽江新团黑谷(LTH)为父本,组配了包括亲本(P)、F1、F2 3个世代的遗传材料.以3个稻瘟病菌系进行苗期室内喷雾接菌鉴定,应用经典遗传学分析方法研究该系谱中的5个供试亲本抗瘟基因的遗传及其与CO39近等基因系中抗瘟基因的等位性关系.结果表明,谷农13、天谷B、福伊B、谷丰B和全丰B等5个亲本的抗瘟性由显性基因控制;谷农13、福伊B、谷丰B和全丰B中都含有2对与CO39近等基因系中Pi-1、Pi-2、Pi-3、Pi-4a、Pi-4b不等位的显性抗稻瘟病基因;天谷B中含有3对显性抗稻瘟病基因,均与Pi-1、Pi-2、Pi-4a、Pi-4b不等位,但有1对与Pi-3等位.
By using Gunong 13,Tiangu B,Fuyi B,Gufeng B and Quanfeng B in rice CMS line pedigree on the breeding male sterile line of rice blast resistance of Fuyi A,Gufeng A and Quanfeng A etc.with blast resistance for female parents,and six rice near-isogenic lines with CO39 for recurrent parent and susceptible control of Lijiangxintuanheigu（LTH） for male parents,materials of 3 generations were matched including parents（P）,F1 and F2.The indoor identification was taken with the method of spraying inoculation of 3 rice blast strains.The genetics of rice blast resistance genes of the 5 tested parents of the pedigree,and the allelism correlation between them and near-isogenic lines of CO39 was analyzed by the method of classical genetics.The results indicated that 5 parents have dominant resistance genes to blast.Gunong13,Fuyi B,Gufeng B and Quanfeng B contain 2 pairs of dominant resistance genes to blast,existing non-allelic relationship with Pi-1,Pi-2,Pi-3,Pi-4a,Pi-4b in NILs of CO39.Tiangu B contains 3 pairs of dominant resistance genes to blast,existing non-allelic relationship with Pi-1,Pi-2,Pi-4a,Pi-4b,while 1 pair of dominant resistance genes exists allelic relationship with Pi-3.This paper revealed the genetic regulations of serial rice parents with rice blast resistance genes,and proposed rice varieties with strong and persistent resistance to rice blast,which have double or more resistance genes,could be bred by marker-assisted selection.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2011年第3期225-230,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金项目
福建省省属公益性项目(2009R10026-2)
福建省重大专项项目(2008NZ0001)资助
关键词
水稻
稻瘟病
抗瘟基因
遗传分析
rice
rice blast
resistance gene to blast
genetic analysis
