摘要
目的:观察耳甲区电针对脂多糖致内毒素血症模型大鼠血清炎性反应因子水平与肺组织核因子κB(NF-κB)表达的影响,探讨耳甲刺激对炎性反应的保护作用机制。方法:SD大鼠随机分为正常对照组、模型组、单纯耳甲电针组、耳甲电针组、迷走神经刺激组、后三里组,每组12只。尾静脉注射脂多糖(5 mg/kg)复制内毒素血症模型。耳甲电针取双侧耳甲区,迷走神经刺激给予左侧颈部迷走神经电刺激,后三里组行双侧"后三里"电针,均为20 min。采用酶联免疫吸附法测定各组大鼠血清肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)水平,采用免疫印迹法测定各组肺组织NF-κB p 65蛋白表达。结果:与正常对照组相比,模型组TNF-α、IL-6水平明显升高(P<0.01),NF-κB p 65表达明显上调(P<0.01);单纯耳甲电针组NF-κB p 65表达明显上调(P<0.01)。与模型组相比,耳甲电针组和迷走神经刺激组TNF-α、IL-6水平明显下降(P<0.01),NF-κB p65表达明显下调(P<0.01);后三里组TNF-α水平明显下降(P<0.05)。与迷走神经刺激组相比,耳甲电针组IL-6水平显著升高(P<0.01),后三里组TNF-α、IL-6水平、NF-κB p 65表达显著升高(P<0.05,P<0.01)。与耳甲电针组相比,后三里组NF-κB p65表达显著升高(P<0.05)。结论:耳甲刺激能降低内毒素血症模型大鼠致炎因子水平,下调NF-κB蛋白表达,其效应与直接刺激迷走神经相似,说明耳甲刺激可能激活了胆碱能抗炎通路,从而启动抗炎效应。
Objective To evaluate the effect of electroacupuncture （EA） of the auricular concha （EA-AC） on serum cytokines contents and pulmonary transcription factor nuclear factor-κB （NF-κB） expression in lipopolysaccharide （LPS） induced en- dotoxaemia rats so as to study its mechanism underlying cholinergic anti-inflammatory efficacy. Methods Male SD rats were randomized into normal control , model （LPS）, simple EA-AC, EA-AC＋ LPS, vagal nerve stimulation （VNS）＋ LPS, and EA-Zu- sanli （ST 36） ＋ LPS groups （n = 12/group）. Endotoxaemia model was duplicated by intravenous （tail vein） injection of LPS （0.5 mL/kg）. Two intradermal needles were inserted into the central sites of the cavity of concha and cymba of auricular concha respectively on each side and stimulated electrically by using an electrical stimulator （ i. e., EA-AO）. VNS was applied to the left cervical vagal nerve, and EA （1 mA, 10 Hz, pulse-width 1 ms） was also applied to bilateral ＂Zusanli＂ （ST 36）, Serum cytokines （TNF-α, IL-6） contents 2 h after modeling were determined by using enzyme linked immunosorbent assay （ELISA）, and pulmonary NF-κB p 65 expression 2 h after modeling was detected by using western blotting. Results Compared with the normal control group, serum TNF-α and IL-6 contents, and pulmonary NF-κB p 65 expression level in the model group were increased significantly （P〈0.01）. In comparison with the model group, serum TNF-α contents in the simple EA-AC, EA-AC＋ LPS, VNS＋ LPS and ST 36 ＋ LPS groups, and serum IL-6 contents and pulmonary NF-κB p 65 expression levels in the simple EA-AC, EA-AC ＋ LPS and VNS ＋ LPS groups were down-regulated considerably （P〈0.05, P〈0.01 ）. Compared with the VNS ＋ LPS group, serum TNF-α and IL-6 contents, and pulmonary NF-κB p 65 expression level in the ST 36 ＋ LPS group were increased significantly （P〈0.05, P-0.01）. In comparison with the EA-AC ＋ LPS group, pulmonary NF-κB p 65 expression level in the ST
出处
《针刺研究》
CAS
CSCD
北大核心
2011年第3期187-192,共6页
Acupuncture Research
基金
国家自然科学基金面上项目(30973798)
北京市自然科学基金重点项目(7111007)
