摘要
目的:讨论PKC在运动性免疫抑制发生过程中的作用。方法:随机将动物分成正常对照组(A组),反复力竭组(B组),中等强度运动组(C组),每组8只。B组动物完成10 d递增运动至反复力竭,C组动物完成中等强度运动10 d,在末次运动后8 h取外周血和脾脏,分离血清测试IFN-γ、IL-4,脾脏分离淋巴细胞,裂解后测试P-PKC含量。结果:B组淋巴细胞PKC磷酸化程度显著低于A组(P<0.01),C组显著高于B组(P<0.01)。B、C组的IFN-γ含量均显著低于A组,而C组显著高于B组(P<0.05)。C组IL-4显著低于A组(P<0.01)和B组(P<0.05)。IFN-γ/IL-4比值B组显著低于A组(P<0.05),C组显著高于B组(P<0.01)。结论:中等强度运动对Th1/Th2平衡和PKC活性影响不大,没有导致运动性免疫抑制,递增负荷运动至反复力竭可使Th1/Th2平衡向Th2方向漂移,使体液免疫相对亢进,表现运动性免疫抑制,这可能与PKC活性显著降低有关。
Purpose： The present study discussed the effects of PKC on the process of mobility immune suppression. Methods： Animals were randomly divided into the normal control group （Group A）, repeated exhaustion group （Group B ） and medium intensity movements （Group C）, with 8 rats in each group. After Group B were treated with activity with increasing training load repeatedly till exhaustion for 10 days and Group C with medium load activity for 10 days, peripheral blood and spleen were obtained from Group B and Group C 8 hours after the last activity. IFN-γ and IL-4 were tested after separating serum, and P-PKC contents were tested after splitting when lymphocytes were separated from spleen. Results： KPC phosphorylation degree of lymphocytes of Group B was remarkably lower than that of Group A （ P 〈0.01 ）, while Group C was significantly higher than Group B （ P 〈 0.01 ）. Compared with Group A, the IFN-γ contents of Group B and C were notably lower, and those of Group C were significantly higher than Group B （P 〈 0.05）. The contents of IL-4 of Group C were notably lower than those of Group A （P 〈0.01 ） and Group B （P 〈0.05）. Compared with Group A, IFN-γ ,1L- 4 ratio contents of Group B were notably lower than Group A （ P 〈 0. 05 ）, while those of Group C were significantly higher than those of Group B. Conclusions： Exercises with moderate intensity have little effect on Thl/Th2 balance and PKC activity and fail to suppress mobility immunity. Repeated exercises with progressively increasing load till exhaustion can make Thl/Th2 balance drift toward Th2 and accentuate relatively body fluid immunity, shown as mobility immune suppression, which is probably related to notable degrade of PCK activity.
出处
《上海体育学院学报》
CSSCI
北大核心
2011年第3期56-59,79,共5页
Journal of Shanghai University of Sport
基金
江西省教育厅科学技术研究项目(GJJ09284)
