摘要
目的:观察脊髓凋亡细胞,明确环磷酰胺对SD大鼠胎仔脊髓神经细胞凋亡作用。方法:大鼠胚胎-胎仔发育毒性试验中,妊娠第10天给予尾静脉注射不同剂量环磷酰胺(7.2 mg/kg和9.0 mg/kg),于妊娠第20天解剖将胎仔脊髓制成病理切片,并行HE和免疫组化染色。结果:实验结果显示实验组脊髓神经出现大量凋亡细胞;与生理盐水组胎仔胸髓和腰髓前角细胞的Bax和Bcl-2表达比较,环磷酰胺低剂量组胎仔胸髓和腰髓前角细胞的Bax表达明显增强,Bcl-2蛋白表达减弱。结论:在大鼠孕第10天给予7.2 mg/kg环磷酰胺尾静脉注射,其对大鼠胎仔脊髓神经细胞凋亡有促进作用。
AIM： To observe the apoptotic cells of fetal spinal cord and define apoptosis of spinal cord by cyclophosphamide and make initial investigation in mechanism. METHODS： During the embryo-fetal development toxicity test,the CP experimental group was injected with CP 7.2 mg/kg and 9.0 mg/kg in the morning of day 10 of gestation;dissected pregnant rats on the day 20 of gestation for fetal spinal cord which were made HE staining and immunohistochemistry.RESULTS：A great quantity of apoptosis cells were observed in experimental group.Compared with negative control physiologic saline group,Bax expression in thoracic cord and isthmus cord anterior horn cell of low-dose CP group increased significantly（P0.01）,and Bcl-2 expression in thoracic cord and isthmus cord anterior horn cell of low-dose CP group are lower（P0.05）.CONCLUSION： These findings suggested that rats injected with CP 7.2 mg/kg in the morning of day 10 of gestation which can aggravate the apoptosis of fetuses＇ spinal nerves.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2011年第4期380-384,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
安全评价
环磷酰胺
致畸试验
脊髓畸形
细胞凋亡
Safety evaluation
Cyclophosphamide（CP）
Teratotoxicity test
Spina bifida
Apoptosis
