摘要
目的探讨白介素13(IL-13)在支气管哮喘发病中的作用以及诱导痰IL-13在哮喘气道炎症中的应用价值。方法 采用酶联免疫吸附实验检测支气管哮喘重度发作(重度组)12例、轻～中度发作(轻～中度组)12例、缓解组10例以及10例健康者(对照组)的诱导痰及血清中IL-13水平,并分别与痰液、血液中白细胞分类以及肺功能指标进行相关性分析。结果支气管哮喘患者IL-13水平在重度组、轻～中度组、缓解组、对照组的诱导痰和血清中的差异均有统计学意义(P<0.05);并且分别与痰液和血液中嗜酸粒细胞分数呈正相关(P<0.05),与肺功能指标呈负相关(P<0.001)。重度发作组痰液和血液的嗜酸粒细胞分数呈正相关(P=0.021)。诱导痰嗜酸粒细胞分数在缓解组和轻～中度组中无直线相关(P>0.05)。结论利用诱导痰检测支气管哮喘患者的气道炎症是一种安全、有效的方法。支气管哮喘患者诱导痰及血清IL-13水平有助于诊断哮喘和判断病情。
Objective To investigate the clinical significance of interleukin-13（IL-13） and the application value of induced sputum technique in the diagnosis of airway inflammation in asthmatic patients. Methods Thirty-four patients with asthma（12 patients at mild and moderate attack stages,12 at sever attack stage,and 10 at remission stage） as well as 10 healthy individuals were enrolled in this study.The levels of IL-13 in induced sputum and serum were determined by enzyme linked immunosorbent assay（ELISA）,and their association with the leukocyte levels and pulmonary function were explored. Results The levels of IL-13 in the induced sputum and serum in these four groups showed significant difference（P〈0.05）.The levels of IL-13 in induced sputum and serum were positively correlated with the ratios of eosinophils/leukocytes（P〈0.05）,and negatively correlated with forced expiratory volume（FEV）,FEV1/FVC,and peak expiratory flow（PEF）（P〈0.001）.There was significant correlation（P=0.021） between the ratios of eosinophils/leukocytes in the induced sputum and in the serum in severe attack group.The ratios of eosinophils/leukocytes of the induced sputum showed no significant defference between the patients at mild/moderate attack stage and those at remission stage（P〉0.05）. Conclusion It was safe and effective to detect airway inflammation using induced sputum technique.The determination of IL-13 levels in the induced sputum and serum is useful for the diagnosis and prognosis of asthma.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第15期1646-1649,共4页
Chinese General Practice
关键词
哮喘
白介素13
炎症
Asthma
Interleukin-13
Inflammation
