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缓释PDGF-BB的纳米珍珠层/聚乳酸/纤维蛋白胶复合支架的制备 被引量:8

Preparation of PDGF-BB releasing nano-nacre/PDLLA/FG composite scaffold
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摘要 [目的]探讨缓释血小板衍生生长因子BB(PDGF-BB)的纳米珍珠层/聚乳酸/纤维蛋白胶复合支架的制备方法及其性能。[方法]采用溶剂浇铸-粒子沥滤法制备聚乳酸支架(S1)和纳米珍珠层/聚乳酸支架(S2),接着将PDGF-BB与纤维蛋白胶混合注入S2,冷冻干燥后制成缓释PDGF-BB的纳米珍珠层/聚乳酸/纤维蛋白胶复合支架(S3)。3种支架通过万能测试机测试抗压强度、扫描电子显微镜观察表面形貌以及阿基米德法测定孔隙率;利用ELISA法检测S3降解过程中PDGF-BB释放量。[结果]3种支架材料均具有良好的三维多孔结构,孔径分别为(330.0±55.6)μm、(322.5±50.8)μm和(303.3±47.0)μm,差异无统计学意义(P>0.05);孔隙率分别为81.0%±1.1%、82.5%±0.8%和80.2%±0.7%,差异无统计学意义(P>0.05);抗压强度分别为(3.6±0.12)MPa、(5.0±0.35)MPa和(5.1±0.26)MPa,S1与S2和S3有显著性差异(P<0.01)。ELISA实验结果表明支架中复合的PDGF-BB释放长达30 d,呈持续缓慢释放。[结论]缓释PDGF-BB的纳米珍珠层/聚乳酸/纤维蛋白胶复合支架为三维多孔结构,具有较好的力学性能,持续缓慢释放PDGF-BB,有望成为一种理想的骨组织工程支架材料。 [ Objective] To investigate the preparation of a platelet- derived growth factor BB (PDGF- BB) releasing nano - nacre/PDLLA/FG composite scaffold. [ Methods ] PDLLA scaffolds ( S1 ) and nano - nacre/PDLLA scaffolds ( S2 ) were fabricated by a simple method of solvent casting and salt leaching, and fibrin glue (FG) mixed with PDGF - BB was infused into the S2 and lyophilized to develop a PDGF - BB releasing nano - nacre/PDLLA/FG composite scaffold ( S2 ) . The morphology and structure properties of the scaffolds were investigated by scanning electron microscopy (SME) and mechanical property testing. The porosity of the scaffold was measured by the Archimedes method. We detected the PDGF - BB releasing amount during the degradation of S3 by ELISA. [ Results ] These scaffolds possessed porous structure, the pore sizes of the 3 scaffolds were 330.0 ± 55.6 μm, 322. 5± 50. 8 μm and 303.3± 47.0 μm. There was no significant difference among them ( P 〉0. 05) . The porosities of the 3 scaffolds were 81.0% ±1. 1% , 82. 5% ±0. 8% and 80. 2% ±0. 7%. There was no significant difference among them (P〉0. 05) . The compressive strength of the 3 scaffolds were 3.6 ±0. 12 MPa, 5.0 ±0. 35 MPa and 5.1±0. 26 MPa. S1 was significanty different from S2 and S3 (P 〈0.01) . In vitro experiments showed that PDGF - BB releasing from the scaffolds sustained for 30 days. [ Conclusion] The PDGF - BB releasing nano - nacre/PDLLA/FG composite scaffold has an appropriate structure, suitable compressive strength and slow release of PDGF - BB. It might be a promising bio-material for bone tissue engineering.
出处 《中国矫形外科杂志》 CAS CSCD 北大核心 2011年第11期943-946,共4页 Orthopedic Journal of China
基金 广东省科技计划项目(编号:2007B031003005)
关键词 血小板衍生生长因子BB 珍珠层 消旋聚乳酸 纤维蛋白胶 支架 platelet - derived growth factor BB, nacre, poly - dl - lactic acid, fibrin glue, scaffold
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