摘要
目的评价KGH-R1、KGH-R2、KGH-R3单克隆抗体(mAb)与胃腺癌组织及正常胃粘膜组织的免疫反应性,为临床应用奠定基础。方法用本实验室制备的三株广谱抗p21 ras mAb KGH-R1、KGH-R2、KGH-R3分别对30例正常胃粘膜组织、30例胃腺癌组织进行免疫组化染色,计算各样本的阳性细胞百分率和组织学评分(HSCORE),比较各组免疫反应性的差异。结果 (1)mAb KGH-R1与83.33%(25/30)的胃腺癌发生免疫反应,阳性样本的阳性细胞百分率为61.26%,HSCORE评分94.52分;与33.33%(10/30)的正常胃粘膜上皮发生免疫反应,阳性样本的阳性细胞百分率为10.00%,HSCORE评分为10.00分。(2)KGH-R2与86.67%(26/30)的胃腺癌发生免疫反应,阳性样本的阳性细胞百分率为86.04%,HSCORE评分155.29分;与23.33%(7/30)的正常胃粘膜上皮发生免疫反应,阳性样本的阳性细胞百分率为7.86%,HSCORE评分为7.86分。(3)KGH-R3与70.00%(21/30)的胃腺癌发生免疫反应,阳性样本的阳性细胞百分率为54.74%,HSCORE评分82.26分;与33.33%(10/30)的正常胃粘膜上皮发生免疫反应,阳性样本的阳性细胞百分率为9.15%,HSCORE评分为9.15分。(4)mAb KGH-R2 HSCORE分值与胃腺癌的浸润深度有关(P<0.05),与组织分化和淋巴结转移无关。结论抗p21 rasmAb KGH-R1、KGH-R2、KGH-R3与胃腺癌具有较好的免疫反应性,只与部分正常胃粘膜呈微弱阳性反应,可开发为治疗性抗体。
Objective To investigate the immunoreactivity of anti-p21ras monoclonal antibody（mAb） KGH-R1,KGH-R2 and KGH-R3 in gastric adenocarcinoma tissues and normal gastric tissues.Methods Immunohistochemical staining was performed in 30 samples from normal gastric tissues and 30 samples from gastric carcinoma tissues using above three monoclonal antibodies prepared in our laboratory.The immunoreactivity of monoclonal antibody KGH-R1,KGH-R2 and KGH-R3 was evaluated by percentage of positive cells and histological score（HSCORE）.Then the findings were compared between the two groups.Results（1）Positive immunostaining of mAb KGH-R1 was found in 83.33%（25/30） of gastric adenocarcinoma,and 61.26% of cancer cells was positive with 94.52 of HSCORE on average.33.33%（10/30） of normal gastric tissues showed positive immunostaining with KGH-R1,but the average percentage of positive cells was 10.00% and the average HSCORE was 10.00.（2） Positive immunostaining of mAb KGH-R2 was found in 86.67%（26/30） of gastric adenocarcinoma,and 86.04% of cancer cells was positive with 155.29 of HSCORE on average.23.33%（7/30） of normal gastric tissues showed positive immunostaining with KGH-R2,but the average percentage of positive cells was 7.86% and the average HSCORE was 7.86.（3） Positive immunostaining of mAb KGH-R3 was found in 70.00%（21/30） of gastric adenocarcinoma,and 54.74% of cancer cells was positive with 82.26 of HSCORE on average.33.33%（10/30） of normal gastric tissues showed positive immunostaining with KGH-R3,but the average percentage of positive cells was 9.15% and the average HSCORE was 9.15.（4） There was a significant correlation between mAb KGH-R2 HSCORE and tumor invasive degree（P0.05）,although mAb KGH-R2 HSCORE had no significant correlations with lymphatic metastasis and histological differentiation.Conclusion mAb anti-p21ras KGH-R1,KGH-R2 and KGH-R3 have high immunoreactivity with gastric adenocarcinoma while only weak positive reaction with partial normal gastric mucosa.There
出处
《西南国防医药》
CAS
2011年第6期581-584,共4页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金项目(30872994)
云南省科技攻关项目(2006SG11)
