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用PCR检测长沙市猪肺炎支原体和猪鼻支原体的感染情况 被引量：11

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摘要 为对长沙市猪支原体肺炎病原流行病学进行初步调查,用PCR方法对从湖南省长沙市5个县(市、区)205头猪中收集的326份病料(205份猪肺和121份猪鼻拭子)分别进行了猪肺炎支原体和猪鼻支原体检测,并对部分基因进行了序列分析。结果发现,猪肺炎支原体检出率为24.4%(50/205),猪鼻支原体检出率为22.3%(27/121);长沙市每个县(市、区)的生猪中均存在这两种支原体,其中长沙县生猪阳性率最高(42.4%和31.5%);序列分析表明,检测出的基因序列与国内外相关序列有97%～100%的同源性。本试验证实了猪肺炎支原体和猪鼻支原体在长沙市猪场的存在。
作者 王贵平 戴玮 贾小雅 丁文格 胡仕凤 王亚楠
机构地区 湖南农业大学动物医学院
出处 《养猪》 2011年第3期81-82,共2页 Swine Production
基金 湖南农业大学东方科技学院创新基金(DFCXY201009)
关键词 PCR 猪肺炎支原体 猪鼻支原体
分类号 S858.28 [农业科学—临床兽医学]
  • 相关文献

参考文献6

  • 1沈青春,宁宜宝,覃青松.猪肺炎支原体的研究进展[J].中国兽药杂志,2003,37(6):26-30. 被引量：56
  • 2Strait E L, Madsen M L, Minion F C.Real-time PCR assays to address genetic diversity among strains of Mycoplasma hyopneumoniae [J]. Journal of Clinical Microbiology, 2008,46 (8): 2491-2498. 被引量：1
  • 3肖国生..胸膜肺炎放线杆菌、猪肺炎支原体和多杀性巴氏杆菌基因芯片检测与分型研究[D].四川农业大学,2006:
  • 4丁芳,宁宜宝.Mhp强弱毒株P97基因的克隆与序列测定[J].畜牧兽医学报,2004,35(6):698-701. 被引量：3
  • 5Lin J H, Chen S P, Yeh K S,et ol. Mycoplasma hyorhinis in Taiwan: diagnosis and isolation of swine pneumonia pathogen [J].Vet Microbiology,2006,15:111-116. 被引量：1
  • 6Caron J, Ouardani M, Dea S. Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasnm hyorhinis infections in pigs by PCR amplification of the p36 and p46 genes[J].J Clin Microbiol,2000,38 (4) :1390-1396. 被引量：1

二级参考文献31

  • 1Leon EA, Madec F, Taylor NM, et al. Seroepidemiology of Mycoplasma hyopneumoniae in pigs from farrow-to-finish farms[J]. Vet Microbiol, 2001,78(4): 331-341. 被引量：1
  • 2Freeman MJ, Lopez-Osuna M, Armstrong CH, et al. Evaluation of the indirect hemagglutination assay as a practical serodiagnostic test for Mycoplasmal pneumonia of swine[J]. Vet Microbiol, 1984, 9(3) : 259-270. 被引量：1
  • 3Bereiter M, Young TF, Joo HS, et al. Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum[J]. Vet Microbiol, 1990,25(2-3) : 177-192. 被引量：1
  • 4Artiushin S, Stipkovits L, Minion FC. Development of polymerase chain reaction primers to detect Mycoplasma hyopneumoniae [ J ].Mol Cell Probes, 1993, 7(5) : 381-385. 被引量：1
  • 5Stemke GW. Gene amplification (PCR) to detect and differentiate Mycoplasmas in porcine Mycoplasmal pneumonia [ J ]. Lett Appl Microbiol, 1997, 25(5) : 327-330. 被引量：1
  • 6Caron J, Ouardani M, Dea S. Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs by PCR amplification of the P36 and P46 genes [ J ]. J Clin Microbiol, 2000 , 38(4) : 1390-1396. 被引量：1
  • 7Wallgren P, Beskow P, FeUstrom C, et al. Porcine lung lesions at slaughter and their correlation to the incidence of infections by Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae during the rearing period [ J ]. Zentralbl Veterinarmed, 1994, 41(7-8) : 441-452. 被引量：1
  • 8Pointon AM, Byrt D, Heap P. Effect of enzootic pneumonia of pigs on growth performance[J]. Aust Vet J, 1985, 62( 1 ) : 13-18. 被引量：1
  • 9Pallares FJ, Gomez S, Munoz A. Evaluation of the zootechnical parameters of vaccinating against swine enzootic pneumonia under field conditions[J]. Vet Rec, 2001, 148(4) : 104-107. 被引量：1
  • 10Rautiainen E, Wallgren P. Aspects of the transmission of protection against Mycoplasma hyopneumoniae from sow to offspring[J]. J Vet Med B Infect Dis Vet Public Health, 2001,48( 1 ) : 55-65. 被引量：1

共引文献57

同被引文献74

  • 1杨贵树,毕峻龙,杨金凤,蒲杨柳,周建琼,尹革芬.猪肺炎支原体YN200901株的分离鉴定[J].动物医学进展,2010,31(9):22-25. 被引量：2
  • 2Su Huang,Ji You Li,Jan Wu,Lin Meng,Cheng Chao Shou Beijing Institute for Cancer Research, Peking University School of Oncology, Beijing 100034. ChinaSu Huang, received B. A from Jiangxi Medical College of China in 1994. Now she is a graduate student pursuing a Ph. D degree at the Peking University School of Oncology..Mycoplasma infections and different human carcinomas[J].World Journal of Gastroenterology,2001,7(2):266-269. 被引量：19
  • 3张锋钢,沈志强,高云英,李浩波,王金良,谢印乾,管宇,刘吉山.猪肺炎支原体的分离及PCR鉴定[J].中国兽医杂志,2007,43(4):21-22. 被引量：12
  • 4曹玉璞 叶立康.支原体与支原体病[M].北京:人民卫生出版社,1997.175-182. 被引量：2
  • 5Elieen L. Thacker. Mycoplasmal Diseases[M]. 赵德明, 张仲秋, 沈建忠译. 猪病学[M].北京:中国农业大学出版社, 9版, 2008.795-802. 被引量：1
  • 6D Maes, J Segales, T Meyns, M Sibila,M Pieters,F Haesebrouck. Control of Mycoplasma hyopneumoniae infections in pigs [J]. Veterinary Microbiology, 2008, 126 ( 4 ) : 297 -309 . 被引量：1
  • 7J H Lin, S P Chen, K S Yeh, C N Weng. Mycoplasma hyorhinis in Taiwan : diagnosis and isolation of swine pneumonia pathogen .[J]. Veterinary Microbiology, 2006, 115(1-3): 111 -116. 被引量：1
  • 8Hans G Drexler and Cord C Uphoff . Mycoplasma contamination of cell cultures : Incidence , sources , effects , detection , elimination , prevention [ J ]. Cytotechnology , 2002 , 39 ( 2 ) : 75 -90. 被引量：1
  • 9M J Freeman , C H Armstrong , L L Sands - Freeman , M Lopez - Osuna . Serological cross - reactivity of porcine Reference antisera to Mycoplasma hyopneumoniae , M . flocculare , M . hyorhinis and M . hyosynoviae indicated by the enzyme - linked immunosorbent assay , complement fixation and indirect hemagglutination tests [ J ]. Canadian journal of comparative medicine,1984 , 48 ( 2 ) : 202 -207. 被引量：1
  • 10J. Caron, M. Ouardani, and S. Dea. Diagnosis and Differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis Infections in Pigs by PCR Amplification of the p36 and p46 Genes[J]. Journal of clinical microbiology, 2000, 38 (4) : 1390 -1396. 被引量：1

引证文献11

二级引证文献25

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2011年 第3期

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