摘要
目的:研究姜黄素(Cur)对化疗药物环磷酰胺(CP)所致微核形成及染色体损伤的影响。方法:昆明小鼠随机分成6组,即正常组,姜黄素组(100mg/kg),模型组,环磷酰胺(CP)+姜黄素低剂量(50mg/kg),环磷酰胺(CP)+姜黄素中剂量(100mg/kg),环磷酰胺(CP)+姜黄素高剂量组(200mg/kg)。正常组即阴性对照组,只喂基础饲料;模型组为环磷酰胺阳性对照组,处死前间隔24h腹腔注射环磷酰胺2次,浓度为50mg/kg;姜黄素组和环磷酰胺+姜黄素组在环磷酰胺给药前4周灌胃给药,每日1次。末次给环磷酰胺24h后将小鼠脱颈椎处死,每只小鼠取股骨作微核观测;另一部分小鼠在处死前2-3h腹腔注射秋水仙素,每10g体质量注射0.04%秋水仙素0.1mL,以积累分裂相,脱颈椎处死后取股骨制备染色体标本。结果:姜黄素对环磷酰胺所引起的微核发生有着显著的抑制作用;姜黄素对环磷酰胺所致的染色体损伤有显著的保护作用。结论:提示姜黄素不仅能改善环磷酰胺在肿瘤治疗中所导致的微核形成,而且能减少由环磷酰胺所诱发的染色体畸变。
Objective： To investigate the effects of curcumin on micronuclei formation frequency and chromosome aberration induced by cyclophosphamide. Methods： Mice were divided into six groups, the control group was treated with saline, model group was treated by intraperitoneal injection of cyclophosphamide （50mg/kg） twice every 24h before sacrifice, curcumin group was gavaged with curcumin suspension at 100mg/kg d and three treated groups were injected by cyclophosphamide combined with curcumin 50 mg/kg cyclophosphamide was given twice by intraperitoneal injection to each mouse every 24h before sacrifice. The dosage of curcumin was 200mg？kg-1？d-1, 100mg？kg-1？d-1, and 50mg？kg-1？d-1 body weight, respectively. The treatment period for all groups was 28 days. Half of all mice were given 0.04% colchicine/10g body weight 2-3h before sacrifice. The micronucleus assay in mice marrow PCE and chromosome aberration assay in mice marrow cells have been used to analyze micronuclei formation frequency and chromosome aberration rate in different groups. Results： The results showed that curcumin not only reduce the micronuclei formation frequency but also down regulate the chromosome aberration rate significantly. Conclusion： These results obtained from our experiments suggested that curcumin had protective effects against micronuclei formation and chromosome aberration.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第6期1373-1375,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
科技部国际合作项目(No.2008DFA31970)
北京中医药大学2008年校级课题资助(No.JYBZZ-JS023)~~
关键词
姜黄素
环磷酰胺
微核
染色体畸变
抗诱变
Curcumin
Cyclophosphamide
Micronuclei
Chromosome aberration
Antimutagenicity
