摘要
为了解我国成都汉族群体和大理白族群体 Ｄ３ Ｓ１５４５ 基因座的遗传多态性，获得中国汉族和大理白族 Ｄ３ Ｓ１５４５ 基因座的群体遗传学数据。采集了 １４８ 名无血缘关系的中国成都汉族个体和９６ 名无血缘关系的大理白族个体的 Ｅ Ｄ Ｔ Ａ 抗凝血样。 Ｃｈｅｌｅｘ 法提取 Ｄ Ｎ Ａ。 Ｐ Ｃ Ｒ 扩增样本 Ｄ Ｎ Ａ，聚丙烯酰胺凝胶水平电泳分型。结果显示：在中国成都汉族群体中， Ｄ３ Ｓ１５４５ 共发现 ９ 个等位基因，等位基因频率为 Ｄ３ Ｓ１５４５８：００６４２； Ｄ３ Ｓ１５４５９：０００３４； Ｄ３ Ｓ１５４５１０：０．００６８； Ｄ３ Ｓ１５４５１１：０．０２０３； Ｄ３ Ｓ１５４５１２：０．１８２４； Ｄ３ Ｓ１５４５１３：０．３０７５； Ｄ３ Ｓ１５４５１４：０３２４３； Ｄ３ Ｓ１５４５１５：０．０８４４； Ｄ３ Ｓ１５４５１６：０．００６８。在大理白族群体中， Ｄ３ Ｓ１５４５ 共发现６ 个等位基因，等位基因频率为 Ｄ３ Ｓ１５４５８：０．００７８１； Ｄ３ Ｓ１５４５１１：０．０２６１； Ｄ３ Ｓ１５４５１２：０．１７１９； Ｄ３ Ｓ１５４５１３：０．３９０６； Ｄ３ Ｓ１５４５１４：０２４４８； Ｄ３
The objective of this study was to understand the distribution of the genetic polymorphisms of STR locus D3S1545 in Chinese populations. EDTA blood specimens were collected from 148 healthy unrelated Han individuals in Chengdu and 96 healthy unrelated Bai individuals in Dali. The DNA samples were extracted using Chelex method, and then they were amplified by PCR technique. PAGE horizontal electrophoresis was used for typing the PCR product. The results there were nine alleles at D3S1545 locus in Chengdu Han population and six alleles at D3S1545 locus in Dali Bai population. The allele frequencies of Chengdu Han population were D3S1545*8:0.0642;D3S1545*9:0.0034;D3S1545*10:0.0068;D3S1545*11:0.0203;D3S1545*12:0.1824; D3S1545*13:0.3075;D3S1545*14:0.3243;D3S1545*15:0.0844;D3S1545*16:0.0068; and 23 genotypes were observed. The allele frequencies of Dali Bai population were D3S1545*8:0.00781;D3S1545*11:0.0261;D3S1545*12:0 1719;D3S1545*13:0.3906;D3S1545*14:0.2448;D3S1545*15:0.0885; and 16 genotypes were observed. The results of test for Hardy Weinberg equilibrium showed that the genotype distributions observed at the populations were correspondent with the expected. These findings suggest that the allele frequencies at D3S1545 locus in Chengdu Hans have not remarkable different from those in the Bai population of Dali. D3S1545 may be a useful marker for paternity test and personal identification of biological stain.
出处
《华西医科大学学报》
CSCD
1999年第3期277-279,共3页
Journal of West China University of Medical Sciences
基金
四川省科技攻关项目
国家自然科学基金
CMB基金
