摘要
在确定了影响航天诱变黑曲霉ZM-8菌株在小麦秸秆为主要原料产纤维素酶的各单因素基础上,采用正交试验分别对其培养基组分和培养条件进行优化。试验结果表明:含氮量和加水量是影响产酶的最主要因素,其产酶培养基组分的最优组合为:最佳氮源为碳酸铵、含氮量1.4%、料水比为1∶2、小麦秸秆粉与麸皮之比为4∶1;培养时间和培养温度是影响产酶的最主要环境因子,其产酶的最佳培养条件为:培养温度为28℃、培养时间为96 h、pH值为6.5、接种量为4%;在优化的培养基配方和培养条件下,其FPU和CMC的酶活分别为6.57 U/g和22.38 U/g。
This experiment was conducted to optimize the fermentating condition of producing cellulose by mutant Aspergillus niger ZM-8.Orthogonal design was applied in this experiment.The results showed that nitrogen concentration and water supplementation were the main factors in medium when producing cellulase.The optimal medium components were as follows： the nitrogen source was（NH4）2CO3 of 1.4 %,the ratio of material to water was 1∶2,the ratio of wheat stalk to bran was 4∶1.The culture time and culture temperature were the main culturing factors in producing cellulase.The optimal cuture conditions were as follow：the culture temperature was 28 ℃,the culture time was 96 h,the initial pH was 6.5 and the inoculation amount was 4 %.Under increased there conditions,the enzyme activities were 6.57 U/g on FPU and 22.38 U/g on CMC.
出处
《中国饲料》
北大核心
2011年第11期17-19,31,共4页
China Feed
基金
甘肃省自然科学研究基金"航天诱变高产纤维素酶菌株黑曲霉的选育及其产酶条件的研究"1010RJZE029)
