摘要
目的： 探讨直接涂片法、分离培养法、聚合酶链反应对淋球菌的诊断价值。方法： 用上述３ 种方法同时对２５８ 例急、慢性淋病患者进行淋球菌检测。结果： 直接涂片、分离培养、 Ｐ Ｃ Ｒ 的阳性率分别为７１７１％ （１８５／２５８）、７０１６％ （１８１／２５８）、９９２２％ （２５６／２５８）， 差异有显著性（ Ｐ＜ ００１）。其中， 男性患者为８４７１％ （１３３／１５７）、７０７０％ （１１１／１５７）、１００％ （１５７／１５７）， 女性患者为５１４９％ （５２／１０１）、６９３０％ （７０／１０１）、９８０２％ （９９／１０１）。结论： 聚合酶链反应较直接涂片、分离培养具有更高的敏感性， 是对淋球菌常规检测的重要补充。
Objective:To study the diagnostic value on the methods of direct smear,isolated culture and polymerase chain reacton(PCR)on Neisseria gonorrhoeae(NG) Methods:The NG in patients was detected by above methods at one time Results:The positive rates of direct smear,isolated culture and PCR were 71 7%(185/258)70 16%(181/258),99 22%(256/258),there was significant difference in three groups( P <0 01) Among them,the positive rates of above methods in male and female were 84 71%(133/157),70 70%(111/157),100%(157/157),and 51 49%(52/101),69 30%(70/101),98 02%(99/101) Conclusion:The method of PCR has higher sensitivity than that of direct smear,isolated culture,and was an important supplement for routine test of NG
出处
《锦州医学院学报》
1999年第4期26-28,共3页
Journal of Jinzhou Medical College
关键词
直接涂片
分离培养
PCR
淋球菌
诊断
direct smear
isolated culture
polymerase chain reaction(PCR)
Neisseria gonorrhoeae(NG)CR has higher sensitivity than that of direct smear,isolated culture,and was an important supplement for routine test of NG Key words:direct smear
isolate
