摘要
目的 研究大黄酸对系膜细胞葡萄糖转运蛋白１（ Ｇ Ｌ Ｕ Ｔ１） 及葡萄糖摄入的影响，探讨大黄酸拮抗 Ｔ Ｇ Ｆβ１ 作用的可能机制。方法 选用体外培养的小鼠肾小球系膜细胞， Ｇ Ｌ Ｕ Ｔ１ ｍ Ｒ Ｎ Ａ表达的检测 采用 Ｎｏｒｔｈｅｒｎ 印迹， 葡萄糖摄入的测定采用２ｄｅｏｘｙ〔３ Ｈ〕 Ｄｇｌｕｃｏｓｅ 摄入法。结果 Ｔ Ｇ Ｆβ１ 显著增加系膜细胞 Ｇ Ｌ Ｕ Ｔ１ ｍ Ｒ Ｎ Ａ 的表达和葡萄糖摄入，大黄酸对正常培养条件下系膜细胞的葡萄糖摄入没有明显影响，但可以拮抗 Ｔ Ｇ Ｆβ１ 增加系膜细胞 Ｇ Ｌ Ｕ Ｔ１ ｍ Ｒ Ｎ Ａ 表达与葡萄糖摄入的作用。结论 Ｔ Ｇ Ｆβ１ 介导系膜细胞葡萄糖代谢的改变以及细胞外基质合成的增加，大黄酸能明显抑制 Ｔ Ｇ Ｆβ１ 所引起的系膜细胞 Ｇ Ｌ Ｕ Ｔ１ 表达与葡萄糖摄入的异常增高。
Objective To explore the possible molecular mechanism that rhein antagonizes the effect of TGFβ1 on glomerular mesangial cells. Methods Cultured C57/SJL mouse mesangial cells were used in the in vitro study. The expression of glucose transporter 1 (GLUT1) mRNA was detected by Northern blot and the ability of glucose uptake was determined by 2 deoxy 〔 3H〕 D glucose uptake assay. Results Rhein had no effect on glucose uptake in mesangial cells cultured in normal glucose concentration. TGFβ1 could upregulate the expression of GLUT1 mRNA and gucose uptake in mesangial cells and this effect was markedly attenuated by the addition of rhein in a dose dependent manner. Conclusion TGFβ1 could upregulate the expression of GLUT1 mRNA and glucose uptake in mesangial cells, leading to excessive glucose consumption and extracellular matrix production in diabetic nephropathy. Rhein antagonized the effect of TGFβ1 in mesangial cells.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1999年第4期229-232,共4页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金
关键词
大黄酸
TGFΒ1
GLUT1
糖尿病肾病
Glucose transporter 1
Rhein
Transforming growth factor β1
Glomerular mesangial cells
