摘要
主要研究了发酵过程中溶氧调控策略对Alcaligenes sp.NX-3产威兰胶的影响。在7.5 L发酵罐中,首先考察了不同通气量对菌体生长、威兰胶浓度、葡萄糖消耗、威兰胶黏度等的影响。结果表明,通气量为1 vvm时,有利于威兰胶浓度的提高。通过研究不同供氧水平对威兰胶发酵的影响,发现较高的溶氧水平有利于威兰胶的合成,但是不利于威兰胶黏度的提高。因此提出了低供氧-中供氧-高供氧、高供氧-中供氧-低供氧2种不同溶氧调控策略。实验结果表明:采用高供氧-中供氧-低供氧策略,能够使威兰胶浓度达到25.4 g/L,葡萄糖对威兰胶的转化率提高到0.51 g/g,威兰胶黏度达到2.700 Pa.s。
The dissolved oxygen control in the production of welan gum by Alcaligenes sp. NX - 3 was investigated. Firstly, based on the analysis of the effect of various aeration rate on cell growth, welan gum concentration, glucose consumption, dissolved oxygen level, and welan gum viscosity, we confirmed that in a 7.5 L bioreactor 1 vvm aeration rate was beneficial for welan gum production. After that, the effect of different oxygen supply levels on the welan gum fermentation was studied. The results indicated that high oxygen supply level could improve the welan gum production, but it was not helpful for welan gum viscosity. Therefore, the two oxygen supply strategies：low-moderate- high oxygen supply level and high-moderate-low oxygen supply level were proposed. The results showed that the latter was better for the welan gum fermentation. Finally, the maximum concentration of welan gum reached 25.4 g/L with a yield of 0.51 g/g and the welan gum viscosity of 2. 700 Pa· s .
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第4期7-11,共5页
Food and Fermentation Industries
基金
江苏省普通高校研究生科技创新计划(CX10B_183Z)
江苏省农业科技自主创新基金(CX(10)226)
国家科技支撑项目“十二五”农村领域(2011BAD23B00)
