摘要
黄原胶是由黄单胞菌属菌分泌的酸性胞外杂多糖,因其具有良好的稳定性和流变性,被广泛用于多种行业。试验在前人研究成果的基础上,以提高黄原胶产量为目的,通过单因素试验确定:在30℃,180 r/min的条件下摇床培养72h,初始碳源浓度6%(蔗糖:淀粉=1:2),接种量10%。提取黄原胶时,加入2%(W/W)的硅藻土,充分震荡10min后离心30min(4 000 r/min),加入1%(W/V)的KNO_3以及3倍体积的混醇(乙醇:异丙醇=3:1)能有效提高提取率。在10L发酵罐中进行小试,产胶率为3.21%。
Xanthan gum（XG） is a kind of acidic extracelluar carbohydrate by Xanthomonas campestris. This polysaccharide is used in many professions due to its characteristic. In order to improve the production rate of XG, the following studies were done. At the condition of 30℃ and 180 r/min, the proper concentration of the carbon source is 6%, the composition of sucrose and starch is optimum carbon source and the optimum inoculum size is 10%. For the conditions of extraction XG, adding diatomite of 2%, agitation for 10 min, centrifugalization for 30 min （4 000 r/min）, adding KNO3 of 1% and alcohol for 3 times volume （ethyl alcohol ： dimethyl carbinol=3 ： 1） could improve the extraction effectively. Finally, the study on the fermentation tank were done, the viscosity of the final fermentation broth is 9 320 MPa-s, the production rate is 3.21%.
出处
《食品工业》
CAS
北大核心
2008年第6期20-23,共4页
The Food Industry
关键词
黄原胶
发酵
提取
xanthangum
fermentation
extraction
