摘要
目的:探讨糖基化终末产物(AGEs)对脂多糖(LPS)介导下的人牙周膜干细胞(HPDLSCs)增殖和骨向分化能力的影响。方法:HPDLSCs培养和鉴定;MTT法检测不同浓度LPS和AGEs对HPDLSCs增殖能力的影响;Real Time-PCR检测10μg/mL LPS和100μg/mL LAGE_S刺激HPDLSCs后白细胞介素-6(IL-6)、碱性磷酸酶(ALP)和Runt-related transcription factor2(RUNX-2)mRNA的表达水平。结果:与正常组比较,10、100μg/mL LPS均抑制HPDLSCs的增殖,50、100、200μg/mL AGEs均抑制HPDLSCs的增殖,100、200μg/mL AGEs比50μg/mL AGEs抑制明显,差异有统计学意义(P<0.05);在成骨诱导下10μg/mL LPS刺激HPDLSCs 6 d和10μg/mL LPS刺激HPDLSCs 3d后再加10μg/mL LPS/100μg/mL AGES共同刺激3d,后者较前者IL-6表达明显增加,ALP和RUNX-2的表达降低,差异有统计学意义(P<0.05)。结论:LPS和AGEs均成浓度依赖性抑制HPDLSCs的增殖能力。AGEs能使LPS介导下的HPDLSCs的炎性因子表达增强,成骨分化能力降低。提示糖尿病可能会加重牙周炎病人的牙周炎炎症程度。
AIM：To investigate the effect of advanced glycation end products（AGEs） on proliferation and osteogenic differentiation of lipopolysaccharide（LPS）-stimulated human periodontal ligament stem cells（HPDLSCs）. METHODS：The effects of LPS and AGEs on the proliferation of HPDLSCs were analyzed by MTT assay.The expressions of interleukin-6（IL-6）,alkaline phosphatase（ALP） and Runt-related transcription factor-2（RUNX-2） mRNA of HPDLSCs which were stimulated by LPS（10μg/mL） and AGEs（100μg/mL） were detected by RT-PCR.RESULTS： MTT assay showed that LPS and AGEs inhibited the proliferation of HPDLSCs in a dose dependent manner. LPS stimulation significantly increased IL-6 expression and inhibited ALP and RUNX-2 expression in HPDLSCs.Co -stimulation of HPDLSCs with LPS and AGEs markedly enhanced the effects of LPS on IL-6,ALP and RUNX-2 expression. CONCLUSION：LPS and AGEs inhibited the proliferation of HPDLSCs in a concentration-dependent man- ner.AGEs can enhance the stimulatory effects of LPS in up-regulating IL-6 expression and the inhibitory effects of LPS in down-regulating osteogenic factors expression.Our results suggest that diabetes may increase the degree of periodontal inflammation in patients with periodontitis.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2011年第4期200-205,共6页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金(30725042
81020108019)
贵州省科技厅自然科学资金(C_393)
关键词
糖基化终末产物
脂多糖
人牙周膜干细胞
advanced glycation end products
lipopolysaccharide
human periodontal ligament stem cell
