摘要
200只21日龄罗斯肉鸡分5个处理组,每组40只,公母各半。处理组1（正对照组）、处理组2（负对照组）,处理组3~5在负对照组日粮基础上各添加一种商业非淀粉多糖（non-starch polysaccharides,NSP）酶制剂。试验期35 d,至56日龄屠宰,测定肠道及内容物消化酶。结果显示：负对照组肉鸡十二指肠和空肠及其内容物的淀粉酶和蛋白酶活性显著小于（P≤0.05）正对照组。处理组3肉鸡十二指肠及其内容物的淀粉酶和蛋白酶活性显著大于（P〉0.05）负对照组,与正对照组相近。处理组3肉鸡空肠内容物的淀粉酶、蛋白酶和脂肪酶活性显著大于（P〈0.05）其它四个处理组。处理组4和5肉鸡空肠及其内容物消化酶活性差异不显著（P〉0.05）。以上结果说明,选用商品NSP酶制剂不能单纯按建议添加量使用,有条件最好实测产品的酶类和酶活性,以便合理有效使用。
Two hundred ROSS broilers of 21 days were designed to five treatment groups in this experiment,including Group 1 and 2（positive and negative treatments）,and Group 3 to 5 with feed additive of commercial non-starch polysaccharide（NSP） enzymes each based on of negative group diet.All broilers were fed 35 days and were killed.The intestine and digesta were sampled for the measurement of digestive enzymes,including amylase,protease and lipase.The results showed that the amylase and protease activities of duodenum,jejunum or both digesta for Group 2（negative treatment） broilers were lower（P 0.05） than those of Group 1（positive treatment）.The amylase and protease activities of duodenum and duodenum digesta for Group 3 were higher than those of Group 2,and were of no difference compared with Group 1.The amylase,protease and lipase activities of jejunum digesta for Group 3 were higher than those of other four groups.There were no significant difference（P 0.05） between Group 4 and 5 for all digestive enzymes in jejunum and jejunum digesta.These findings suggested the addition of commercial NSP enzymes in broiler diets cannot be applied with the recommended dose because of the diversity of NSP enzyme species and（or） enzyme activities and the autivity of NSP enzyme its should be measured so as to apply it reasonably and effectively.
出处
《家畜生态学报》
2011年第2期64-68,共5页
Journal of Domestic Animal Ecology
基金
科技部科技人员服务企业行动项目(2009GJF30046)
云南省科技攻关项目(2008LA006)
昆明市科技局项目(08G100112)
云南省创新型企业试点项目
关键词
商业非淀粉多糖酶制剂
消化酶
肉鸡
commercial non-starch polysaccharide enzyme
digestive enzyme
broiler
