摘要
目的:观察调节性T细胞在星形胶质瘤患者体内的表达情况,观察其与星形胶质瘤发展的关系。方法:收集河北医科大学第四医院神经外科在2008年9月至2010年6月期间收治的星形胶质瘤患者,共92例,其中男性54例,女性38例,平均年龄51.4岁,均为初次发病。收集8例行颞叶切除术的癫痫患者为对照组。应用流式细胞术检测观察CD4^+CD25^+F0XP3^+调节性T细胞(Treg)在胶质瘤组织中及患者外周血中的浸润情况,应用免疫酶联反应方法(ELISA)测定转化生长因子β1(TGFβ1)在外周血中的表达,应用SPSSl3.0软件进行统计学分析处理。结果:星形胶质瘤患者外周血(9.34±2.13)%和肿瘤组织(24.17±2.54)%中的Treg细胞明显增多,均显著高于对照组(0.67%±0.12%,24.17%±2.54%,P<0.05),并且Treg细胞的含量随着肿瘤级别的升高而增加,WH0Ⅳ级患者外周血和肿瘤组织中的Treg细胞含量(8.34%±2.13%,35.45%±2.47%)明显高于WH0Ⅲ和WH0Ⅱ级(P<0.05)。ELISA结果显示:星形胶质瘤患者外周血中TGFβ1的含量明显增高,并与外周血中的Treg细胞的数量明显呈正相关(r=0.564,P=0.001)。结论:Treg细胞在星形胶质瘤患者的外周血和肿瘤组织的浸润明显增多,并与肿瘤的恶性程度有关,其数量的增长与TGFβ1的表达相关。Treg细胞的增多能够抑制机体抗肿瘤免疫,促进了星形胶质瘤的发展,其具有成为胶质瘤免疫治疗靶点的潜在价值。
Objective： To investigate the expression of regulatory T lymphocytes in human astrocytoma and its correlation with the progression of astrocytoma. Methods： Data were collected from astrocytoma patients treated in The 4th Hospital Affiliated to Hebei Medical University between September 2008 and June 2010. The expression ofCD4 CD25 FOXP3 regulatory T lymphocytes （ Treg ） in the tumor tissue and blood were detected by flow cytometry （ FCM ）. The expression of transforming growth factor β1 （ TGFβ1 ） was detected by ELISA test. Data were analyzed by SPSS software 13.0. Results： A total of 92 patients （ 54 males and 38 females, with a mean age of 51.4 years ） who underwent surgical treatment were divided into three groups according to the pathological grade （ 42 grade Ⅳ cases, 21 grade Ⅲ cases, 29 grade Ⅱ cases. The controled brain tissue was obtained from 8 patients who underwent a temporal Iobectomy for seizures. There were significant differences in Treg cells between astrocytoma patients and the controls （ 10.67 % 0.12 % ） both in the brain tissue （ 24.17 % ±2.54 % ） and in the blood （ 9.34 % ±2.13 % ） （ P〈 0.01 ）. The expression of Treg in astrocytoma was positively correlated with the tumor grade （ P 〈 0.05 ）. The expression of TGF β1 in the blood of glioma patients was positively correlated with Treg cells （ r = 0.564, P = 0. 001 ）. Conclusion： Our study showed significant increase of Treg cells in the tumor tissue and peripheral blood of patients with astrocytoma. The role of Tregs in the tumor biology of gliomas might have potential therapeutic implications.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2011年第8期459-462,共4页
Chinese Journal of Clinical Oncology
