摘要
采用乳化交联法制备载药壳聚糖微球,在聚乙烯醇与甲醛缩醛化过程中加入载药壳聚糖微球从而制备聚乙烯醇/载药壳聚糖微球敷料,研究了载药壳聚糖微球含量对海绵的微观形貌、吸水率、膨胀率、力学性能及体外释放的影响。结果表明:聚乙烯醇/载药壳聚糖微球复合海绵具有连通的孔隙结构,增加壳聚糖微球含量使孔径变小,从而使海绵的吸水率、膨胀率下降,但拉伸强度和压缩强度增加;此外,增加壳聚糖微球含量使头孢拉定药物释放速率增加,释放时间增长。从毒性评级结果比较,此种材料的毒性级别均是0-1级,为无毒,符合生物相容性要求。白兔鼻腔术后观察表明,壳聚糖改性缩醛化PVA海绵具有消炎、抗粘连、促进鼻黏膜愈合和功能恢复的作用。利用聚乙烯醇/载药壳聚糖微球复合海绵作为敷料应用于负压引流装置后,可以为患者的肉芽组织提供最佳的生长环境,肉芽组织生长良好,基本可以覆盖暴露的骨、肌腱,填满缺损区。
In order to prepare and apply the polyvinyl alcohol/drug-loaded chitosan microspheres composite wound dressing,we first prepared chitosan microspheres by emulsion cross-linking method,and then added chitosan microspheres into the reactants during the acetalization of polyvinyl alcohol and formaldehyde.We further studied the morphology,water absorption,swelling degree,mechanical properties and in vitro release of the sponge with different amount of chitosan microspheres.The results showed that polyvinyl alcohol/drug-loaded chitosan composite sponge has porous structure with connectionism.Increasing the amount of chitosan microspheres would make the apertures smaller,so that the water absorption and the swelling of sponge decreased,but the tensile strength and compressive strength increased.With the increase of the amount of chitosan microspheres,the drug absorption of cefradine and the release rate increase,and the release time become longer.With the results of toxicity grade of 0 to 1,this type of composite sponge is non-toxic and meets the requirement of biocompatibility.The observation of rabbit nasal cavity after surgical operation suggested that polyvinyl acetal sponge modified with the chitosan has antiphlogistic,hemostatic and non-adherent characteristic,and can promote the healing and recovering of the nasalmucosa.After using this composite material,best growing surroundings for patients＇ granulation tissue were provided.Exposed bone and tendon were covered well with granulation tissue.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2011年第2期381-386,共6页
Journal of Biomedical Engineering
基金
中科院广州化学所纤维素化学重点实验室开放课题资助(LCLC-2008-05)
暨南大学引进人才项目资助(51207019)
关键词
壳聚糖微球
聚乙烯醇
敷料
Chitosan microspheres
Polyvinyl alcohol
Wound dressing
